Van Deusen M A, Gentile D A, Skoner D P
Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pennsylvania.
Ann Allergy Asthma Immunol. 1997 Mar;78(3):259-64. doi: 10.1016/S1081-1206(10)63178-X.
Previous investigations have documented that a sodium, potassium adenosine triphosphatase (Na+,K+ ATPase) enzyme inhibitor is bound to the platelet membrane, displaced from the platelet membrane by freezing, and present in the plasma of subjects with allergic rhinitis. Others have shown that stimulation of Na+,K+ ATPase is an important early event in mitogen-induced activation of peripheral blood mononuclear cells.
The purpose of this study was to determine whether the Na+,K+ ATPase enzyme inhibition observed in the platelets of subjects with allergic rhinitis also extends to peripheral blood mononuclear cells.
Na+,K+ ATPase activity of a particulate fraction of sonicated peripheral blood mononuclear cells was determined by spectrophotometry in asymptomatic adults with and without allergic rhinitis.
The mean Na+,K+ ATPase activity of peripheral blood mononuclear cells expressed as nanomoles per microgram protein per minute (nM/ microgram protein/ min) +/-1 standard deviation of the subjects with allergic rhinitis (n = 14) was 1.04 +/- 1.01, while that of the control subjects (n = 12) was 3.57 +/- 1.60 (P < or = .001). In contrast, when the peripheral blood mononuclear cell membranes were frozen and then thawed prior to assay, the mean Na+,K+ ATPase activity for the subjects with allergic rhinitis (n = 24) was 5.33 +/- 2.62, while that of the control subjects (n = 23) was 1.12 +/- 1.24 (P < or = .001). Samples from a subset of subjects (n = 5) were assayed for both pre-freezing and post-freezing Na+,K+ ATPase activity. The freezing process was associated with a striking increase in Na+,K+ ATPase levels of subjects with allergic rhinitis (4.42 +/- 2.06) but a decrease in those of the control subjects (-3.89 +/- 0.95; P < or = .001).
These data demonstrate that peripheral blood mononuclear cells from subjects with allergic rhinitis, like platelets, possess a membrane-bound Na+,K+ ATPase inhibitor that is displaced from the membrane by freezing. In vivo Na+,K+ ATPase inhibition could have significant effects on the activation and function of peripheral blood mononuclear cells in subjects with allergic rhinitis.
先前的研究已证明，钠钾腺苷三磷酸酶（Na +，K + ATP酶）抑制剂与血小板膜结合，通过冷冻从血小板膜上解离，并存在于过敏性鼻炎患者的血浆中。其他人则表明，刺激Na +，K + ATP酶是丝裂原诱导外周血单核细胞活化的重要早期事件。
本研究的目的是确定在过敏性鼻炎患者血小板中观察到的Na +，K + ATP酶抑制是否也扩展到外周血单核细胞。
通过分光光度法测定有和没有过敏性鼻炎的无症状成年人中超声处理的外周血单核细胞颗粒部分的Na +，K + ATP酶活性。
以每分钟每微克蛋白质的纳摩尔数（nM /微克蛋白质/分钟）表示的过敏性鼻炎患者（n = 14）外周血单核细胞的平均Na +，K + ATP酶活性+/- 1标准差为1.04 +/- 1.01，而对照组（n = 12）为3.57 +/- 1.60（P≤0.001）。相比之下，当外周血单核细胞膜在测定前冷冻然后解冻时，过敏性鼻炎患者（n = 24）的平均Na +，K + ATP酶活性为5.33 +/- 2.62，而对照组（n = 23）为1.12 +/- 1.24（P≤0.001）。对一部分受试者（n = 5）的样本进行冷冻前和冷冻后Na +，K + ATP酶活性测定。冷冻过程与过敏性鼻炎患者Na +，K + ATP酶水平的显著增加（4.42 +/- 2.06）相关，但与对照组的降低（-3.89 +/- 0.95; P≤0.001）相关。
这些数据表明，过敏性鼻炎患者的外周血单核细胞与血小板一样，具有膜结合的Na +，K + ATP酶抑制剂，其通过冷冻从膜上解离。体内Na +，K + ATP酶抑制可能对过敏性鼻炎患者外周血单核细胞的活化和功能产生重大影响。
