Reiss R A, James A A
Department of Molecular Biology and Biochemistry, University of California at Irvine, 92717, USA.
Insect Mol Biol. 1993;2(1):25-32. doi: 10.1111/j.1365-2583.1993.tb00122.x.
A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila melanogaster gene, DmGST-2. The conceptual translation product also shows similarity to the pi family of vertebrate GSTs. Northern analysis reveals multiple and abundant transcription products in larval, pupal and adult RNA preparations. Southern analyses of genomic DNA and hybridizations in situ to polytene chromosomes both suggest that there are multiple members of the GST gene family in Anopheles gambiae.
从成年雌性冈比亚按蚊文库中分离出一个长度为1456个核苷酸的cDNA（D7 - 8B），根据推导的氨基酸序列，它被鉴定为谷胱甘肽S - 转移酶（GST）基因家族的成员。该基因AgGST2 - 1编码的蛋白质与黑腹果蝇基因DmGST - 2有57%的同源性。其概念翻译产物也与脊椎动物GSTs的pi家族相似。Northern分析显示，在幼虫、蛹和成虫的RNA制剂中有多个丰富的转录产物。基因组DNA的Southern分析以及与多线染色体的原位杂交均表明，冈比亚按蚊中存在GST基因家族的多个成员。
