Cooper S J, Hewitt G M
School of Biological Sciences, University of East Anglia, Norwich.
Insect Mol Biol. 1993;2(3):185-94. doi: 10.1111/j.1365-2583.1993.tb00138.x.
Sequence data from a non-coding nuclear DNA segment (Cpnl-1) was used to investigate the divergence between Spanish and French subspecies of the grasshopper Chorthippus parallus which form a hybrid zone in the Pyrenees. The spatial distribution of eighteen Cpnl-1 sequence haplotypes from eighteen French and fifteen Spanish populations and phylogenetic analyses using both distance and parsimony procedures provide strong evidence that the Pyrenean hybrid zone was formed by postglacial secondary contact and that the French subspecies arose by range expansion from a Balkan or Italian refugium. In addition, the data provide evidence for subdivision between grasshoppers from Northern Spain and those from Central and Pyrenean Spanish populations.
来自非编码核DNA片段（Cpnl-1）的序列数据被用于研究在比利牛斯山脉形成杂交带的草蜢Chorthippus parallus西班牙亚种和法国亚种之间的分化。来自18个法国种群和15个西班牙种群的18个Cpnl-1序列单倍型的空间分布，以及使用距离法和简约法程序进行的系统发育分析提供了强有力的证据，表明比利牛斯杂交带是由冰期后二次接触形成的，并且法国亚种是通过从巴尔干或意大利避难所的范围扩张而产生的。此外，数据还为西班牙北部的草蜢与西班牙中部和比利牛斯种群的草蜢之间的细分提供了证据。
