Miadonna A, Gibelli S, Lorini M, Tedeschi A, Oddera S, Rossi G A, Crimi E
Unità di Allergia e Immunopatologia Respiratoria, Università di Milano, IRCCS Ospedale Maggiore Policlinico, Italy.
Lung. 1997;175(3):195-209. doi: 10.1007/pl00007567.
Recently, much attention has been given to the possible role of lymphocytes and their soluble products in causing and maintaining allergic inflammation. The aim of this study was to assess the production of mRNAs for interleukins (IL) in bronchoalveolar lavage (BAL) cells obtained from allergic asthmatics after challenge with the relevant allergen in the period between early and late reactions. We evaluated BAL fluid cells obtained from six asthmatic subjects and four nonatopic controls. Challenge was performed with the relevant allergen. BAL fluid cells were obtained by fiberoptic bronchoscopy and bronchoalveolar lavage. To detect mRNA encoding each cytokine in BAL cells we used a reverse transcriptase polymerase chain reaction method. We evaluated IL-1 alpha, -2, -4, -5, -6, -13, and granulocyte-macrophage colony-stimulating factor (GM-CSF), and interferon-gamma (IFN-gamma). mRNAs for IL-1 alpha, -2, -4, -5, and IFN-gamma were detected in all of the atopic subjects; mRNAs for IL-6 and GM-CSF were found in five asthmatics; and mRNA for IL-13 was found in one patient only. In contrast, no mRNAs for IL-2, -4, -5, -6, -13, and GM-CSF were detected in the nonatopic healthy controls; mRNA for IL-1 alpha was found in one out of four normal subjects; and mRNA for IFN-gamma was evidenced in two of four subjects. The cellular environment in BAL fluids from allergic asthmatics before the clinical appearance of the late airway reaction shows an unrestricted expression of mRNA for cytokines. The local cytokine milieu could have an important role in the modulation of bronchial inflammation and in the appearance of allergic symptoms.
最近，淋巴细胞及其可溶性产物在引发和维持过敏性炎症中可能发挥的作用受到了广泛关注。本研究的目的是评估在早发反应和迟发反应期间，用相关过敏原激发后，从过敏性哮喘患者获得的支气管肺泡灌洗（BAL）细胞中白细胞介素（IL）的mRNA产生情况。我们评估了从6名哮喘患者和4名非特应性对照者获得的BAL液细胞。用相关过敏原进行激发。通过纤维支气管镜检查和支气管肺泡灌洗获取BAL液细胞。为了检测BAL细胞中编码每种细胞因子的mRNA，我们使用了逆转录聚合酶链反应方法。我们评估了IL-1α、-2、-4、-5、-6、-13、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和干扰素-γ（IFN-γ）。在所有特应性受试者中均检测到IL-1α、-2、-4、-5和IFN-γ的mRNA；在5名哮喘患者中发现了IL-6和GM-CSF的mRNA；仅在1例患者中发现了IL-13的mRNA。相比之下，在非特应性健康对照者中未检测到IL-2、-4、-5、-6、-13和GM-CSF的mRNA；在4名正常受试者中有1名发现了IL-1α的mRNA；在4名受试者中有2名发现了IFN-γ的mRNA。在迟发性气道反应临床表现出现之前，过敏性哮喘患者BAL液中的细胞环境显示细胞因子mRNA的表达不受限制。局部细胞因子环境可能在调节支气管炎症和过敏性症状的出现中起重要作用。
