Wahle P, Reimann S
Lehrstuhl für Allgemeine Zoologie und Neurobiologie, Ruhr-Universität, Fakultät für Biologie, Bochum, Germany.
Brain Res Dev Brain Res. 1997 Mar 17;99(1):72-86. doi: 10.1016/s0165-3806(96)00208-8.
The postnatal development of the cat pretectum has been analysed with in situ hybridization and immunohistochemistry with the aim to establish the time course of morphological and neurochemical maturation of parvalbumin (PARV), calbindin-D28k (CALB), and glutamic acid decarboxylase (GAD) expressing neuronal populations. At birth, PARV-ir retinal afferents to the pretectum have already formed distinct termination zones which appear as 3 clusters separated by intercluster regions in coronal sections. The clusters contain two sets of large neurons expressing either PARV or CALB. The two sets of neurons differ in the time at which they grow rapidly. Both sets reach the adult size at P38. PARV-ir retinal fibers contact dendrites of large PARV-negative, and thus presumably CALB-ir neurons. A population of smaller CALB-ir neurons appears within the clusters during the second postnatal week. In intercluster regions, small PARV-ir and CALB-ir neurons are present at birth, but increase in number during development. Only PARV-ir intercluster neurons increase in size between P4 and P38. GAD neurons are present dorsal to the clusters and in intercluster regions from P0 onwards. However, within the clusters GAD neurons do not appear until the second postnatal week. The different onset of marker expression and cellular growth patterns suggest the existence of several populations of CaBP-ir excitatory and inhibitory neurons in the pretectum. The final complement of inhibitory neurons is not present until the second postnatal week. These developmental processes may correlate with the slow maturation of the pretectal motion processing system and the cortico-pretectal projection.
已通过原位杂交和免疫组织化学分析了猫前顶盖的产后发育情况，目的是确定表达小白蛋白（PARV）、钙结合蛋白-D28k（CALB）和谷氨酸脱羧酶（GAD）的神经元群体在形态和神经化学成熟方面的时间进程。出生时，前顶盖的PARV免疫反应性视网膜传入纤维已经形成了明显的终末区，在冠状切片中表现为3个簇，被簇间区域分隔开。这些簇包含两组分别表达PARV或CALB的大神经元。这两组神经元在快速生长的时间上有所不同。两组在出生后38天达到成年大小。PARV免疫反应性视网膜纤维与大的PARV阴性神经元的树突接触，因此推测这些大神经元是CALB免疫反应性神经元。在出生后第二周，簇内出现了一群较小的CALB免疫反应性神经元。在簇间区域，出生时就存在小的PARV免疫反应性和CALB免疫反应性神经元，但在发育过程中数量增加。只有PARV免疫反应性簇间神经元在出生后4天到38天之间体积增大。从出生后第0天起，GAD神经元就存在于簇的背侧和簇间区域。然而，直到出生后第二周，簇内才出现GAD神经元。标记物表达和细胞生长模式的不同起始时间表明，前顶盖中存在几群CaBP免疫反应性兴奋性和抑制性神经元。抑制性神经元的最终补充直到出生后第二周才出现。这些发育过程可能与前顶盖运动处理系统和皮质-前顶盖投射的缓慢成熟有关。
