Chuang Y H, Chiang B L, Chou C C, Hsieh K H
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Clin Exp Allergy. 1997 Mar;27(3):315-24.
It has been well documented that environmental factors such as antigenpresenting cells and related cytokines could affect the development of T helper cells.
The purpose of this study is to investigate the effect of different adjuvants on T cell development.
Ovalbumin (OVA) combined with aluminum hydroxide (Alum) plus pertussis toxin (PT) or complete Freund's adjuvant (CFA) were used to sensitize mice; the production of IgG and IgE anti-OVA antibodies was then followed. In addition, OVA-specific proliferative responses and cytokine production by spleen cells were also investigated.
The data showed that the adjuvants themselves could modify the pattern of immune response: (1) IgG2a anti-OVA antibody was higher in mice sensitized with OVA + CFA compared to that of mice sensitized with OVA + Alum + PT; (2) the ratio of IFN-gamma/IL-4 produced by OVA-stimulated spleen cells was higher in mice sensitized with OVA + CFA than that of mice sensitized with OVA + Alum + PT: (3) increased percentage of gamma delta T cells was noted in the peritoneal exudate cells of OVA + CFA immunized mice; and (4) the immune response of mice sensitized with OVA + Alum + PT was inhibited by the adoptively transferred ascitic cells from OVA + CFA immunized mice.
In general, the data suggested higher IgG2a and the ratio of IFN-gamma/IL-4 was noted in mice sensitized with OVA + CFA. Further elucidation of the regulatory mechanism of allergen-specific T helper cells development and exploration of possible agents for immunotherapy might shed light on the management of atopic diseases.
有充分文献记载，诸如抗原呈递细胞和相关细胞因子等环境因素可影响辅助性T细胞的发育。
本研究旨在探讨不同佐剂对T细胞发育的影响。
用卵清蛋白（OVA）联合氢氧化铝（明矾）加百日咳毒素（PT）或完全弗氏佐剂（CFA）致敏小鼠；随后检测抗OVA抗体IgG和IgE的产生。此外，还研究了OVA特异性增殖反应以及脾细胞产生的细胞因子。
数据表明，佐剂本身可改变免疫反应模式：（1）与用OVA+明矾+PT致敏的小鼠相比，用OVA+CFA致敏的小鼠中抗OVA抗体IgG2a更高；（2）用OVA刺激的脾细胞产生的IFN-γ/IL-4比值，用OVA+CFA致敏的小鼠高于用OVA+明矾+PT致敏的小鼠；（3）在OVA+CFA免疫小鼠的腹腔渗出细胞中，γδT细胞百分比增加；（4）用OVA+明矾+PT致敏的小鼠的免疫反应受到来自OVA+CFA免疫小鼠的过继转移腹水细胞的抑制。
总体而言，数据表明用OVA+CFA致敏的小鼠中IgG2a及IFN-γ/IL-4比值更高。进一步阐明变应原特异性辅助性T细胞发育的调节机制以及探索可能的免疫治疗药物，可能为特应性疾病的管理提供线索。
