Tooyama I, Sasaki K, Oomura Y, Li A J, Kimura H
Institute of Molecular Neurobiology, Shiga University of Medical Science, Otsu, Japan.
Exp Gerontol. 1997 Jan-Apr;32(1-2):171-9. doi: 10.1016/s0531-5565(96)00071-x.
We examined the effects of chronic administration of acidic fibroblast growth factor (aFGF) on memory and choline acetyltransferase (ChAT) immunoreactivity in the forebrain of senescence-accelerated mice (SAMP8 strain). Subcutaneous injection of aFGF (aFGF group) or saline vehicle (saline group) once a week into SAMP8 was begun at three weeks after birth and continued for nine months. In the passive avoidance test, the retained latency was significantly longer in the aFGF group than in the saline group. In the Morris test, the mean latency to climb on a platform was significantly shorter in the aFGF group than in the saline group. The number of ChAT-positive neurons in the forebrain septum was greater in the aFGF group than in the saline group, and was at the level of that in the control mouse strain (SAMR1). The intensity of ChAT staining in the aFGF group appeared slightly weaker than in SAMR1 but significantly stronger than in the saline group. The results indicate that the effect of aFGF on memory function in SAMP8 may be related to the preservation of function in septal cholinergic cells.
我们研究了长期给予酸性成纤维细胞生长因子（aFGF）对衰老加速小鼠（SAMP8品系）前脑记忆和胆碱乙酰转移酶（ChAT）免疫反应性的影响。从出生后三周开始，每周一次皮下注射aFGF（aFGF组）或生理盐水（生理盐水组）到SAMP8小鼠体内，持续九个月。在被动回避试验中，aFGF组的记忆潜伏期明显长于生理盐水组。在莫里斯试验中，aFGF组爬上平台的平均潜伏期明显短于生理盐水组。aFGF组前脑隔区ChAT阳性神经元的数量多于生理盐水组，且与对照小鼠品系（SAMR1）的水平相当。aFGF组ChAT染色强度似乎略弱于SAMR1，但明显强于生理盐水组。结果表明，aFGF对SAMP8记忆功能的影响可能与隔区胆碱能细胞功能的保留有关。
