Boulanger A, Asselin I, Roy R, Tremblay J P
Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Québec, Canada.
Transplantation. 1997 Mar 27;63(6):893-9. doi: 10.1097/00007890-199703270-00016.
Myoblasts obtained from donors histoincompatible for several non-major histocompatibility complex antigens (i.e., including minor histocompatibility antigens) and from syngeneic donors were transplanted without any immunosuppression into the muscles of male dystrophic C57BL/10J mdx/mdx mice. Myoblasts from syngeneic mice resulted in the formation of a high percentage of dystrophin-positive fibers 16 weeks after the transplantation. There was no evidence of a cellular immune reaction against the donor myoblasts, i.e., no infiltration by CD4 or CD8 lymphocytes and no increased expression of granzyme B and interferon-gamma mRNAs. Transplantation of myoblasts obtained from donors histoincompatible only for non- major histocompatibility complex antigens produced a transient increase of dystrophin-positive fibers at 4 weeks after transplantation for some donor strains but not for others. For donor strains that did produce an increase at 4 weeks, the number of dystrophin-positive fibers was reduced 16 weeks after the transplantation. There was evidence of a cellular immune reaction-infiltration by CD4 and by CD8 lymphocytes and increased expression of granzyme B and interferon-gamma mRNAs. Transplantation of myoblasts obtained from male C57BL/10J +/+ mice into female C57BL/10J mdx/mdx mice also led to the presence of only a few dystrophin-positive fibers with the same signs of cellular immune reaction. In this later case, the cellular immune response was attributed to the H-Y minor antigens. Finally, antibodies against fetal calf serum were detected after both syngeneic and nonsyngeneic transplantations, indicating that the culture medium may also be a source of antigens. In mice, the presence of these antibodies against culture medium did not reduce the success of a first syngeneic transplantation.
从与几种非主要组织相容性复合体抗原（即包括次要组织相容性抗原）组织不相容的供体以及同基因供体获得的成肌细胞，在没有任何免疫抑制的情况下被移植到雄性营养不良的C57BL/10J mdx/mdx小鼠的肌肉中。同基因小鼠的成肌细胞在移植后16周导致形成了高比例的抗肌萎缩蛋白阳性纤维。没有证据表明对供体成肌细胞存在细胞免疫反应，即没有CD4或CD8淋巴细胞浸润，也没有颗粒酶B和干扰素-γ mRNA的表达增加。仅从与非主要组织相容性复合体抗原组织不相容的供体获得的成肌细胞移植，对于某些供体品系在移植后4周产生了抗肌萎缩蛋白阳性纤维的短暂增加，但其他品系则没有。对于在4周时确实出现增加的供体品系，移植后16周抗肌萎缩蛋白阳性纤维的数量减少。有细胞免疫反应的证据——CD4和CD8淋巴细胞浸润以及颗粒酶B和干扰素-γ mRNA的表达增加。将雄性C57BL/10J +/+小鼠的成肌细胞移植到雌性C57BL/10J mdx/mdx小鼠中也仅导致少数抗肌萎缩蛋白阳性纤维的存在以及相同的细胞免疫反应迹象。在这后一种情况下，细胞免疫反应归因于H-Y次要抗原。最后，在同基因和非同基因移植后均检测到了抗胎牛血清抗体，表明培养基也可能是抗原的来源。在小鼠中，这些针对培养基的抗体的存在并没有降低首次同基因移植的成功率。
