Audinat E, Lambolez B, Cauli B, Ropert N, Perrais D, Hestrin S, Rossier J
Laboratoire de Neurobiologie, CNRS-URA 2054, ESPCI, Paris, France.
J Physiol Paris. 1996;90(5-6):331-2. doi: 10.1016/s0928-4257(97)87911-9.
The biochemical and functional characteristics of the AMPA subtype of the glutamate receptors expressed by pyramidal and non-pyramidal neurons of the neocortex have been studied in acute slices by means of single-cell RT-PCR and fast applications of glutamate on outside-out patches. Our results suggest that the predominant expression of the flop splice variants of the GluR1-4 AMPA subunits contributes to the faster desensitization of these receptors in non-pyramidal neurons compared to pyramidal cells where flip variants of GluR1-4 are dominant. Alternative splicing of AMPA receptors may therefore play an important role in regulating synaptic function in a cell-type specific manner.
通过单细胞逆转录聚合酶链反应（RT-PCR）以及在外侧外翻膜片上快速施加谷氨酸，在急性脑片中研究了新皮质锥体细胞和非锥体细胞所表达的谷氨酸受体AMPA亚型的生化和功能特性。我们的结果表明，与GluR1-4翻转变体占主导的锥体细胞相比，GluR1-4 AMPA亚基的翻转剪接变体在非锥体细胞中的主要表达导致了这些受体更快的脱敏。因此，AMPA受体的可变剪接可能以细胞类型特异性方式在调节突触功能中发挥重要作用。
