Kaur S, Saxena R N, Mallick B N
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Neurosci Lett. 1997 Feb 21;223(2):105-8. doi: 10.1016/s0304-3940(97)13410-3.
The aminergic neurons in the locus coeruleus are known to cease firing during rapid eye movement sleep. Since electrical stimulation of locus coeruleus reduced, while carbachol stimulation increased rapid eye movement sleep and gamma-aminobutyric acid (GABA) neurons as well as terminals are present in the locus coeruleus, we hypothesized that GABA may be involved for cessation of locus coeruleus neuronal firing during rapid eye movement sleep. Under surgical anaesthesia male Wistar rats (250-300 g) with bilateral guide cannulae targeting locus coeruleus were prepared for chronic sleep-wakefulness recording. Electroencephalogram (EEG), electrooculogram (EOG), electromyogram (EMG) were recorded in normal, after 250 nl saline and after picrotoxin (250 ng in 250 nl) injection bilaterally into the locus coeruleus. The results showed that mean duration per episode of rapid eye movement sleep was significantly reduced, although its frequency of generation/h was not significantly affected. This study suggests that GABA in locus coeruleus is involved in tonic regulation of rapid eye movement sleep and the action is mediated through GABAA receptor.
已知蓝斑中的胺能神经元在快速眼动睡眠期间停止放电。由于对蓝斑的电刺激会减少快速眼动睡眠，而卡巴胆碱刺激会增加快速眼动睡眠，并且蓝斑中存在γ-氨基丁酸（GABA）神经元及其终末，我们推测GABA可能参与了快速眼动睡眠期间蓝斑神经元放电的停止。在手术麻醉下，制备了双侧引导套管靶向蓝斑的雄性Wistar大鼠（250 - 300克）用于慢性睡眠 - 觉醒记录。在正常情况下、双侧蓝斑注射250微升生理盐水后以及注射苦味毒（250纳克溶于250微升）后记录脑电图（EEG）、眼电图（EOG）和肌电图（EMG）。结果显示，尽管每小时快速眼动睡眠的发生频率没有受到显著影响，但每次快速眼动睡眠发作的平均持续时间显著缩短。这项研究表明，蓝斑中的GABA参与了快速眼动睡眠的紧张性调节，且该作用是通过GABAA受体介导的。
