Hadas E, Mazur T
Department of Biology and Medical Parasitology, K. Marcinkowski University of Medical Sciences, Poznan, Poland.
Parasitol Res. 1997;83(3):296-9. doi: 10.1007/s004360050250.
The aim of the present study was to examine the biosynthesis of prostaglandins and to investigate factors conditioning their biosynthesis in pathogenic and nonpathogenic strains of Acanthamoeba spp. We established that the activity of the synthase of prostaglandins was almost identical in pathogenic and non-pathogenic strains and that the synthesis of endoperoxide prostaglandins was similar to that of other organisms up to the point at which prostaglandin H2 was produced. The course of biosynthesis in vitro can be activated by various compounds such as glutathione, albumin, and p-chloromercuribenzoic acid (p-CMB), which are either activators or inhibitors of the enzymes. We suggest that the course of biosynthesis of prostaglandins in vivo is most probably activated by tissues or constitutional liquids surrounding the parasites.
本研究的目的是检测棘阿米巴属致病和非致病菌株中前列腺素的生物合成，并研究影响其生物合成的因素。我们发现，致病菌株和非致病菌株中前列腺素合酶的活性几乎相同，并且环内过氧化物前列腺素的合成在生成前列腺素H2之前与其他生物体相似。体外生物合成过程可被各种化合物激活，如谷胱甘肽、白蛋白和对氯汞苯甲酸（p-CMB），它们要么是酶的激活剂，要么是抑制剂。我们认为，体内前列腺素的生物合成过程很可能被寄生虫周围的组织或体液激活。
