Reed M W, Panyutin I G, Hamlin D, Lucas D D, Wilbur D S
Epoch Pharmaceuticals Inc., Bothell, Washington 98021, USA.
Bioconjug Chem. 1997 Mar-Apr;8(2):238-43. doi: 10.1021/bc970016n.
A rapid and efficient method for the synthesis of 125I-labeled oligodeoxynucleotides ([125I]ODNs) is described. The key intermediates are tributylstannylbenzamide-modified ODNs (Sn-ODNs). Reaction conditions are described for the preparation of 5'-modified Sn-ODNs. Treatment with NaI and chloramine T gave conversion to the desired I-ODN, which was easily isolated by reversed phase chromatography. Thermal denaturation (Tm) studies showed that hybridization properties were not disturbed by the 4-iodobenzamide modification. An [125I]ODN was prepared and characterized by hybridization to 32P-labeled DNA targets. Sequence specific cleavage of the target DNA strand by 125I was measured.
描述了一种快速有效的合成125I标记寡脱氧核苷酸（[125I]ODNs）的方法。关键中间体是三丁基锡苯甲酰胺修饰的ODNs（Sn-ODNs）。描述了制备5'-修饰的Sn-ODNs的反应条件。用NaI和氯胺T处理可转化为所需的I-ODN，通过反相色谱法可轻松分离。热变性（Tm）研究表明，4-碘苯甲酰胺修饰不会干扰杂交特性。制备了一种[125I]ODN，并通过与32P标记的DNA靶标杂交进行了表征。测量了125I对靶DNA链的序列特异性切割。
