Suppr超能文献

位点特异性蛋白质 - DNA 复合物稳定性或不稳定性的传递。

The transmission of stability or instability from site specific protein-DNA complexes.

作者信息

Wartell R M

出版信息

Nucleic Acids Res. 1977 Aug;4(8):2779-97. doi: 10.1093/nar/4.8.2779.

DOI:10.1093/nar/4.8.2779
PMID:909792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342608/
Abstract

Theoretical calculations were made to determine the influence of side specific 'melting' and 'stabilizing' proteins on the thermal stability of nearby base pairs (bp). A DNA sequence 999bp. long containing the 123 bp. lactose operon control region in the center was examined. Melting curves of base pairs near the binding sites of the catabolite activator protein, CAP, the lactose repressor, and RNA polymerase were calculated in the absence and presence of each protein. The empirical loop entropy model of the helix-coil transition of DNA was employed. Calculations show that melting and stabilizing proteins alter the tm of base pairs 20 to 100 bp-away. The magnitude and range of the effect is strongly influenced by the base pair composition and sequence of the protein site and the immediately adjacent DNA regions.

摘要

进行了理论计算，以确定侧翼特异性“解链”和“稳定”蛋白对附近碱基对（bp）热稳定性的影响。研究了一个长度为999bp的DNA序列，其中心包含123bp的乳糖操纵子控制区域。在每种蛋白质存在和不存在的情况下，计算了分解代谢物激活蛋白（CAP）、乳糖阻遏物和RNA聚合酶结合位点附近碱基对的解链曲线。采用了DNA螺旋-卷曲转变的经验环熵模型。计算结果表明，解链蛋白和稳定蛋白会改变距离其20至100bp处碱基对的解链温度（tm）。这种效应的大小和范围受到蛋白质结合位点以及紧邻的DNA区域的碱基对组成和序列的强烈影响。

相似文献

1
The transmission of stability or instability from site specific protein-DNA complexes.位点特异性蛋白质 - DNA 复合物稳定性或不稳定性的传递。
Nucleic Acids Res. 1977 Aug;4(8):2779-97. doi: 10.1093/nar/4.8.2779.
2
The catabolite activator protein stabilizes its binding site in the E. coli lactose promoter.分解代谢物激活蛋白可稳定其在大肠杆菌乳糖启动子中的结合位点。
Nucleic Acids Res. 1985 Oct 25;13(20):7483-98. doi: 10.1093/nar/13.20.7483.
3
Analysis of tet operator-TET repressor complexes by thermal denaturation studies.通过热变性研究分析四环素操纵子-四环素阻遏物复合物
Nucleic Acids Res. 1982 Oct 11;10(19):6085-97. doi: 10.1093/nar/10.19.6085.
4
Raman spectroscopy of DNA-metal complexes. II. The thermal denaturation of DNA in the presence of Sr2+, Ba2+, Mg2+, Ca2+, Mn2+, Co2+, Ni2+, and Cd2+.DNA-金属络合物的拉曼光谱。II. Sr2+、Ba2+、Mg2+、Ca2+、Mn2+、Co2+、Ni2+和Cd2+存在下DNA的热变性
Biophys J. 1995 Dec;69(6):2623-41. doi: 10.1016/S0006-3495(95)80133-5.
5
Co-operative interactions between the catabolite gene activator protein and the lac repressor at the lactose promoter.分解代谢物基因激活蛋白与乳糖启动子处的乳糖阻遏物之间的协同相互作用。
J Mol Biol. 1990 Jul 20;214(2):381-96. doi: 10.1016/0022-2836(90)90188-R.
6
Initiation of in vitro mRNA synthesis from the wild-type lac promoter.从野生型乳糖启动子起始体外mRNA合成。
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4394-8. doi: 10.1073/pnas.72.11.4394.
7
Non-specific DNA binding of genome regulating proteins as a biological control mechanism: I. The lac operon: equilibrium aspects.作为一种生物控制机制的基因组调控蛋白的非特异性DNA结合：I. 乳糖操纵子：平衡方面
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4808-12. doi: 10.1073/pnas.71.12.4808.
8
DNA melting and promoter clearance by eukaryotic RNA polymerase I.真核生物RNA聚合酶I介导的DNA解链与启动子清除
J Mol Biol. 2000 May 26;299(1):75-89. doi: 10.1006/jmbi.2000.3743.
9
Binding of lactose repressor to poly d(A-T) : OD AND CD melting of the complex.乳糖阻遏蛋白与聚d(A-T)的结合：复合物的OD和CD熔解
Nucleic Acids Res. 1975 Mar;2(3):303-18. doi: 10.1093/nar/2.3.303.
10
A direct real-time spectroscopic investigation of the mechanism of open complex formation by T7 RNA polymerase.对T7 RNA聚合酶形成开放复合物机制的直接实时光谱研究。
Biochemistry. 1996 Dec 10;35(49):15715-25. doi: 10.1021/bi960729d.

引用本文的文献

1
Mechanism for transcriptional action of cyclic AMP in Escherichia coli: entry into DNA to disrupt DNA secondary structure.环磷酸腺苷在大肠杆菌中转录作用的机制：进入DNA以破坏DNA二级结构。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4011-5. doi: 10.1073/pnas.78.7.4011.

本文引用的文献

1
Thermodynamics of the helix-coil transition of the alternating copolymer of deoxyadenylic acid and deoxythymidylic acid.脱氧腺苷酸与脱氧胸苷酸交替共聚物螺旋-线圈转变的热力学
J Mol Biol. 1969 Jun 28;42(3):577-80. doi: 10.1016/0022-2836(69)90244-7.
2
Statistical thermodynamics of nucleic acid melting transitions with coupled binding equilibria.具有耦合结合平衡的核酸熔解转变的统计热力学
Biopolymers. 1971 Nov;10(11):2147-60. doi: 10.1002/bip.360101110.
3
Free energy of imperfect nucleic acid helices. II. Small hairpin loops.不完全核酸螺旋的自由能。II. 小发夹环
J Mol Biol. 1973 Feb 5;73(4):497-511. doi: 10.1016/0022-2836(73)90096-x.
4
The nucleotide sequence of the lac operator.乳糖操纵基因的核苷酸序列。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4. doi: 10.1073/pnas.70.12.3581.
5
Lac repressor binding to non-operator DNA: detailed studies and a comparison of eequilibrium and rate competition methods.乳糖阻遏蛋白与非操纵基因DNA的结合：详细研究以及平衡和速率竞争方法的比较
J Mol Biol. 1972 Dec 30;72(3):671-90. doi: 10.1016/0022-2836(72)90184-2.
6
Stability of ribonucleic acid double-stranded helices.核糖核酸双链螺旋的稳定性
J Mol Biol. 1974 Jul 15;86(4):843-53. doi: 10.1016/0022-2836(74)90357-x.
7
Recursion relation generation of probability profiles for specific-sequence macromolecules with long-range correlations.具有长程相关性的特定序列大分子概率分布的递归关系生成
Biopolymers. 1974;13(9):1859-71. doi: 10.1002/bip.1974.360130916.
8
Conformational transitions of cyclic adenosine monophosphate receptor protein of Escherichia coli. A fluorescent probe study.大肠杆菌环磷酸腺苷受体蛋白的构象转变。荧光探针研究。
Biochemistry. 1974 Jun 4;13(12):2567-72. doi: 10.1021/bi00709a015.
9
Purification and DNA-binding properties of the catabolite gene activator protein.分解代谢基因激活蛋白的纯化及DNA结合特性
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1222-5. doi: 10.1073/pnas.68.6.1222.
10
Specific binding of CAP factor to lac promoter DNA.CAP因子与乳糖操纵子启动子DNA的特异性结合。
Nature. 1975 Aug 21;256(5519):672-4. doi: 10.1038/256672a0.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验