Calore E E, Maeda M Y, Cavaliere M J, Pereira S M, Shih L W, Pereira G M, de Melo J R
Instituto de Infectologia Emilio Ribas São Paulo, Brasil.
Minerva Ginecol. 1997 Mar;49(3):59-62.
Thirty three biopsies of the uterine cervix were studied by the AgNOR method, that identifies the nucleolar organizer regions. These comprised 9 cases of cervicitis (with or without squamous metaplasia), 9 cases of cervical intraepithelial neoplasia grade I (CIN I), 8 CIN II and 10 CIN III. A hundred cells were counted and classified according to the number of AgNOR dots. We use a more practical and fast method of AgNOR dots counting in cervical intraepithelial neoplasia, in that we exclude the two basal layers and count only cells with 4 or more dots. Statistically significant differences for AgNOR dots were found between cervicitis or CIN I and CIN II cases (p < 0.02) and between CIN II and CIN III cases (p < 0.001). No statistical difference was found between the cases of cervicitis and CIN I. It was concluded that this method of AgNOR counting can be useful in the identification and classification of individual cases intraepithelial neoplasia and their differentiation from eventual difficult cases of cervicitis.
采用鉴别核仁组成区的AgNOR方法对33例子宫颈活检组织进行了研究。这些病例包括9例宫颈炎（伴或不伴鳞状化生）、9例宫颈上皮内瘤变I级（CIN I）、8例CIN II和10例CIN III。根据AgNOR点的数量对100个细胞进行计数和分类。我们在宫颈上皮内瘤变中采用了一种更实用、快速的AgNOR点计数方法，即排除两层基底层，仅对有4个或更多点的细胞进行计数。在宫颈炎或CIN I与CIN II病例之间（p < 0.02）以及CIN II与CIN III病例之间（p < 0.001）发现AgNOR点存在统计学显著差异。宫颈炎与CIN I病例之间未发现统计学差异。得出的结论是，这种AgNOR计数方法有助于宫颈上皮内瘤变个体病例的识别和分类，以及将其与最终可能难以鉴别的宫颈炎病例区分开来。
