一类新型大环内酯类化合物，即由栖气糖丝菌亚种抗生糖丝菌产生的糖癌菌素。I. 分类学、发酵、分离及生物学特性。

A family of novel macrocyclic lactones, the saccharocarcins produced by Saccharothrix aerocolonigenes subsp. antibiotica. I. Taxonomy, fermentation, isolation and biological properties.

作者信息

Horan A C, Shearer M C, Hedge V, Beyazova M L, Brodsky B C, King A, Berrie R, Cardaci K, Nimeck M

机构信息

Schering Plough Research Institute, Department of Microbial Products, Kenilworth, NJ 07033, USA.

出版信息

J Antibiot (Tokyo). 1997 Feb;50(2):119-25. doi: 10.7164/antibiotics.50.119.

DOI:10.7164/antibiotics.50.119

PMID:9099220

Abstract

A nocardioform actinomycete, SCC 1886, isolated from a soil sample collected in Ohio was found to produce, in fermentation, six novel macrocyclic lactones, the saccharocarcins. The producing culture was identified as Saccharothrix aerocolonigenes subsp. antibiotica based on the formation of fragmenting substrate mycelia, aerial mycelia that coalesce to form aerial colonies, whole-cell hydrolysates that contain meso-diaminopimelic acid, galactose and rhamnose and physiological comparisons to type species of the genus. Peak production of the saccharocarcins occurred after 95 hours of fermentation in a starch rich medium. The compounds were isolated from the fermentation broth by solvent extraction and purified by HPLC. Isolated compounds were active against Micrococcus luteus, Staphylococcus aureus and Chlamydia trachomatis; none were cytotoxic at concentrations up to 1.0 microgram/ml.

摘要

从俄亥俄州采集的一份土壤样本中分离出的一种诺卡氏放线菌SCC 1886，经发酵发现可产生六种新型大环内酯类化合物——糖癌菌素。基于形成断裂的基内菌丝体、融合形成气生菌落的气生菌丝体、含有内消旋二氨基庚二酸、半乳糖和鼠李糖的全细胞水解产物以及与该属模式种的生理比较，将产生该化合物的培养物鉴定为产气糖丝菌抗生素亚种。在富含淀粉的培养基中发酵95小时后，糖癌菌素产量达到峰值。通过溶剂萃取从发酵液中分离出这些化合物，并通过高效液相色谱法进行纯化。分离出的化合物对藤黄微球菌、金黄色葡萄球菌和沙眼衣原体具有活性；在浓度高达1.0微克/毫升时均无细胞毒性。