Stiles J, McDermott M, Bigsby D, Willis M, Martin C, Roberts W, Greene C
Department of Small Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
Am J Vet Res. 1997 Apr;58(4):338-42.
To use a nested polymerase chain reaction (PCR) to detect feline herpesvirus (FHV-1) DNA in conjunctiva or cornea from clinically normal cats and cats with conjunctivitis or corneal sequestra.
Conjunctival snip biopsy specimens from 50 cats with conjunctivitis and 50 clinically normal cats; 28 keratectomy specimens from 26 cats with sequestra, and 13 specimens from clinically normal cats.
Tissue specimens were digested, and FHV-1 DNA was amplified, using a double round of PCR. Products were visualized by use of agarose gel electrophoresis.
Polymerase chain reaction was positive in 27 of 50 (54%) conjunctival specimens from cats with conjunctivitis and 6 of 50 (12%) specimens from clinically normal cats. Difference in the results between cats with conjunctivitis and clinically normal cats was statistically significant. Polymerase chain reaction was positive in 5 of 28 (18%) corneal specimens from cats with sequestra and 6 of 13 (46%) clinically normal cats. Distribution of positive results between clinically normal cats and those with sequestra was not significant.
Cats with conjunctivitis were more likely to have a positive PCR result than were clinically normal cats, making it likely that FHV-1 was associated with the disease state. Herpesvirus DNA could not be detected in most corneas from cats with sequestra.
Polymerase chain reaction is a useful clinical test for identifying FHV-1 DNA in cats with conjunctivitis, yielding greater sensitivity over that of current available tests. Herpesvirus may be less of a cause of corneal sequestration in the commonly affected breeds, Himalayan and Persian, than other factors, such as lagophthalmos or corneal metabolic defects.
采用巢式聚合酶链反应（PCR）检测临床正常猫以及患有结膜炎或角膜翳的猫的结膜或角膜中的猫疱疹病毒（FHV - 1）DNA。
50只患有结膜炎的猫和50只临床正常猫的结膜剪取活检标本；26只患有角膜翳的猫的28份角膜切除术标本，以及13只临床正常猫的标本。
组织标本经消化后，通过两轮PCR扩增FHV - 1 DNA。产物通过琼脂糖凝胶电泳进行可视化分析。
在50只患有结膜炎的猫的结膜标本中，27只（54%）PCR呈阳性，而在50只临床正常猫的标本中，6只（12%）呈阳性。患有结膜炎的猫与临床正常猫的检测结果差异具有统计学意义。在28只患有角膜翳的猫的角膜标本中，5只（18%）PCR呈阳性，而在13只临床正常猫中，6只（46%）呈阳性。临床正常猫和患有角膜翳的猫之间阳性结果的分布无显著差异。
患有结膜炎的猫比临床正常猫更有可能出现PCR阳性结果，这表明FHV - 1可能与疾病状态相关。在大多数患有角膜翳的猫的角膜中未检测到疱疹病毒DNA。
PCR是一种用于鉴定患有结膜炎的猫中FHV - 1 DNA的有用临床检测方法，其敏感性高于目前可用的检测方法。在常见的受影响品种，如喜马拉雅猫和波斯猫中，疱疹病毒可能比其他因素，如兔眼症或角膜代谢缺陷，更少成为角膜翳的病因。
