Gomez S P, Handler A M
Center for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture, Gainesville, Florida 32608, USA.
Insect Mol Biol. 1997 May;6(2):165-71. doi: 10.1111/j.1365-2583.1997.tb00084.x.
Transformation of a Drosophila virilis white mutant host strain was attempted using a hobo vector containing the D. melanogaster mini-white(+) cassette (H[w(+), hawN]) and an unmodified or heat shock regulated hobo transposase helper. Two transformant lines were recovered with the unmodified helper (HFL1), one containing only the white(+) marked vector, and a sibling line containing the vector as well as an HFL1 helper integration. An approximate total transformation frequency of 1% is deduced. A high frequency of wing and eye morphology mutants were also observed, suggesting that hobo may have mobilized a related element in D. virilis. The data reaffirms a relatively low transformation vector activity for the hobo transposon in D. virilis; however, nearly full interspecific expression white(+) marker supports its possible function in other species as well.
尝试使用含有黑腹果蝇微小白色（+）盒（H[w(+), hawN]）的hobo载体以及未修饰或热休克调节的hobo转座酶辅助因子，对粗壮果蝇白色突变宿主菌株进行转化。使用未修饰的辅助因子（HFL1）获得了两个转化株系，一个仅含有白色（+）标记载体，另一个同胞株系既含有该载体又含有HFL1辅助因子整合体。推断出的总转化频率约为1%。还观察到高频的翅和眼形态突变体，这表明hobo可能在粗壮果蝇中激活了一个相关元件。数据再次证实了hobo转座子在粗壮果蝇中的转化载体活性相对较低；然而，几乎完全的种间白色（+）标记表达支持了它在其他物种中也可能具有的功能。
