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识别5'-GGATG-3'的BstF5I-1 DNA甲基转移酶的一级结构和链特异性

Primary structure and strand specificity of BstF5I-1 DNA methyltransferase which recognizes 5'-GGATG-3'.

作者信息

Degtyarev S Kh, Netesova N A, Abdurashitov M A, Shevchenko A V

机构信息

SibEnzyme, Novosibirsk, Russia.

出版信息

Gene. 1997 Mar 18;187(2):217-9. doi: 10.1016/s0378-1119(96)00752-4.

DOI:10.1016/s0378-1119(96)00752-4
PMID:9099883
Abstract

The gene for BstF5I-1 DNA-methyltransferase (MTase) from Bacillus stearothermophilus F5 (a FokI isoschizomer, recognizing 5'-GGATG-3') was cloned and its nucleotide (nt) sequence was determined. Analysis of deduced amino acid (aa) sequence shows that M x BstF5I-1 belongs to D21 class of MTases and has a little homology with M x FokI. M x BstF5I-1 modifies only the upper strand of recognition sequence (5'-GGATG-3').

摘要

克隆了嗜热脂肪芽孢杆菌F5（一种FokI同裂酶，识别序列为5'-GGATG-3'）的BstF5I-1 DNA甲基转移酶（MTase）基因，并测定了其核苷酸（nt）序列。对推导的氨基酸（aa）序列分析表明，Mx BstF5I-1属于MTases的D21类，与Mx FokI有少许同源性。Mx BstF5I-1仅修饰识别序列（5'-GGATG-3'）的上链。

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