Shin D W, Lee Y H, Rho T J
Department of Parasitology, College of Medicine, Chungnam National University, Taejon, Korea.
Korean J Parasitol. 1997 Mar;35(1):55-62. doi: 10.3347/kjp.1997.35.1.55.
The molecular weight 30 kDa membrane protein of Toxoplasma gondii (Toxoplasma 30 kDa) apparently conserved in most strains of T. gondii and sera of infected hosts. The present study aimed to elucidate Toxoplasma 30 kDa as a useful diagnostic antigen for serodiagnosis of toxoplasmosis by ELISA and for induction of protective immunity. Murine spleen cells immunized with the membrane antigen of T. gondii were fused with mouse Sp2/O-Ag14 myeloma cells. Out of 8 clones selected, five were IgG2b, the others belonged to IgG1 and IgG2a. The 30 kDa antigen was distributed mainly on the surface membrane of tachyzoites by indirect fluorescence method. Murine peritoneal macrophages which were activated by 30 kDa antigen produced more amounts of NO2 compared with crude antigen-treated group, however there were no significant differences in toxoplamacidal activity between the two groups. Higher specificity of Toxoplasma 30 kDa antigen was recognized for serodiagnosis of toxoplasmosis than the crude antigen. From these results, Toxoplasma 30 kDa antigen enhances the cytotoxic effect of macrophages as well as a more reliable means for the serodiagnosis of toxoplasmosis by ELISA.
弓形虫分子量30 kDa的膜蛋白（弓形虫30 kDa）在大多数弓形虫菌株和感染宿主血清中明显保守。本研究旨在阐明弓形虫30 kDa作为通过酶联免疫吸附测定（ELISA）进行弓形虫病血清学诊断以及诱导保护性免疫的有用诊断抗原。用弓形虫膜抗原免疫的小鼠脾细胞与小鼠Sp2/O-Ag14骨髓瘤细胞融合。在所选的8个克隆中，5个为IgG2b，其他属于IgG1和IgG2a。通过间接荧光法，30 kDa抗原主要分布在速殖子的表面膜上。与粗抗原处理组相比，由30 kDa抗原激活的小鼠腹腔巨噬细胞产生更多量的二氧化氮（NO2），然而两组之间的杀弓形虫活性没有显著差异。与粗抗原相比，弓形虫30 kDa抗原在弓形虫病血清学诊断中具有更高的特异性。从这些结果来看，弓形虫30 kDa抗原增强了巨噬细胞的细胞毒性作用，并且是通过ELISA进行弓形虫病血清学诊断的更可靠手段。
