Liu H, Ding R, Jiang S, Yang J, Cheny H, Lu D, Zi L, Wu F
Department of Haematology, Affiliated Hospital of Nantong Medical College, Jiangsu, China.
Tokushima J Exp Med. 1996 Dec;43(3-4):107-11.
To describe the suppressive effect of sera from patients with aplastic anemia (AA) on heamatopoietic progenitor cells and explore further the features of serum inhibitor, we first examined the inhibiting activity of the serum on the colony formation of burst-forming units of erythrocyte (BFU-E) and colony-forming units of granulocyte/macrophage (CFU-GM). The potent inhibiting activity was demonstrated in six of 20 patients. Five of the six patients were severe AA (SAA). The suppressive effect did not change after elimination of monocyles and lymphocytes from target MNC. The haematopoietic inhibitory activity of sera from three of the six patients was almost totally neutralized by the MoAb against gamma-IFN. All these three patients were SAA. These data suggest that inhibitor of haematopoietic progenitor cells is present in most of SAA patients. In some patients, the inhibitor was gamma-IFN, which play an important role in the pathogenesis of SAA.
为描述再生障碍性贫血（AA）患者血清对造血祖细胞的抑制作用，并进一步探究血清抑制剂的特征，我们首先检测了血清对红细胞爆式集落形成单位（BFU-E）和粒细胞/巨噬细胞集落形成单位（CFU-GM）集落形成的抑制活性。20例患者中有6例表现出较强的抑制活性。这6例患者中有5例为重型AA（SAA）。从靶单个核细胞（MNC）中去除单核细胞和淋巴细胞后，抑制作用未改变。6例患者中3例血清的造血抑制活性几乎被抗γ-干扰素单克隆抗体（MoAb）完全中和。这3例患者均为SAA。这些数据表明，大多数SAA患者存在造血祖细胞抑制剂。在一些患者中，抑制剂为γ-干扰素，其在SAA发病机制中起重要作用。
