胸膜肺炎放线杆菌外膜脂蛋白（omlA）编码基因的遗传多样性。
Genetic diversity of the genes encoding the outer membrane lipoprotein (omlA) of Actinobacillus pleuropneumoniae.
作者信息
Osaki M, Sato Y, Tomura H, Ito H, Sekizaki T
机构信息
National Institute of Animal Health, Ibaraki, Japan.
出版信息
J Vet Med Sci. 1997 Mar;59(3):213-5. doi: 10.1292/jvms.59.213.
An allelic variation of the genes encoding the protective outer membrane lipoprotein (omlA) in Actinobacillus pleuropneumoniae was investigated with polymerase chain reaction (PCR)-restriction fragment length polymorphisms. Primers for PCR were selected from a conserved sequence compared between the omlA genes of A. pleuropneumoniae serotypes 1 and 5a. A DNA fragment of 970 bp was amplified from the genomic DNA of all 12 serotypes of A. pleuropneumoniae. The amplified DNA sequence specifically hybridized under a low stringent condition to the cloned omlA gene of A. pleuropneumoniae. Digestion of the amplified DNA with the enzymes either HinfI or VspI yielded specific polymorphic patterns, allowing discrimination of all serotypes into five distinct groups.
利用聚合酶链反应（PCR）-限制性片段长度多态性技术，对胸膜肺炎放线杆菌中编码保护性外膜脂蛋白（omlA）的基因的等位基因变异进行了研究。PCR引物是从胸膜肺炎放线杆菌1型和5a型的omlA基因之间比较的保守序列中选择的。从胸膜肺炎放线杆菌的所有12个血清型的基因组DNA中扩增出一个970 bp的DNA片段。扩增的DNA序列在低严格条件下与胸膜肺炎放线杆菌克隆的omlA基因特异性杂交。用HinfI或VspI酶消化扩增的DNA产生了特定的多态性模式，可将所有血清型分为五个不同的组。
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