Nylander A G, von Friesen C P, Monstein H J, Yamada H, Chen D, Boketoft A, Håkanson R
Department of Pharmacology, University of Lund, Sweden.
Pharmacol Toxicol. 1997 Mar;80(3):147-51. doi: 10.1111/j.1600-0773.1997.tb00388.x.
Porta-caval shunting enhances the trophic effects of cholecystokinin (CCK)-A receptor activation on the pancreas and of CCK-B receptor activation on the ECL cells in the oxyntic mucosa of the rat. The aim of the present study was to study the expression of CCK-A and CCK-B receptor mRNA after porta-caval shunting. Different doses of sulfated CCK-8 (CCK-8s) were administered to porta-caval shunting rats and sham-operated rats, 4 weeks after the operations. The pancreatic wet weight and DNA content were measured and the ECL cells in the oxyntic mucosa were counted after four days of continuous subcutaneous infusion. Total RNA was isolated from pancreas and oxyntic mucosa for Northern blot analysis of CCK-A and CCK-B receptor mRNA. Porta-caval shunting per se did not affect plasma CCK level nor the weight or DNA content of the pancreas, but resulted in increased number of ECL cells despite the fact that the serum gastrin concentration was reduced. The trophic response of the pancreas to low doses of CCK-8s was greater in porta-caval shunted rats than in sham-operated rats. Porta-caval shunted rats displayed an increased CCK-A receptor mRNA concentration in the pancreas (after stimulation with CCK-8s) and an increased CCK-B receptor mRNA concentration in the oxyntic mucosa. In conclusion, the porta-caval shunting-evoked enhancement of the trophic effect of CCK-A receptor activation on the pancreas and of CCK-B receptor activation on the ECL cells is associated with enhanced expression of CCK-A receptor mRNA in the pancreas and of CCK-B receptor mRNA in the oxyntic mucosa.
门腔分流术增强了胆囊收缩素（CCK）-A受体激活对大鼠胰腺的营养作用以及CCK-B受体激活对大鼠胃黏膜壁细胞中肠嗜铬样（ECL）细胞的营养作用。本研究的目的是研究门腔分流术后CCK-A和CCK-B受体mRNA的表达。术后4周，对门腔分流大鼠和假手术大鼠给予不同剂量的硫酸化CCK-8（CCK-8s）。连续皮下输注4天后，测量胰腺湿重和DNA含量，并对胃黏膜中的ECL细胞进行计数。从胰腺和胃黏膜中分离总RNA，用于CCK-A和CCK-B受体mRNA的Northern印迹分析。门腔分流术本身并不影响血浆CCK水平，也不影响胰腺的重量或DNA含量，但尽管血清胃泌素浓度降低，却导致ECL细胞数量增加。门腔分流大鼠胰腺对低剂量CCK-8s的营养反应比假手术大鼠更大。门腔分流大鼠胰腺中（经CCK-8s刺激后）CCK-A受体mRNA浓度增加，胃黏膜中CCK-B受体mRNA浓度增加。总之，门腔分流引起的CCK-A受体激活对胰腺的营养作用增强以及CCK-B受体激活对ECL细胞的营养作用增强与胰腺中CCK-A受体mRNA和胃黏膜中CCK-B受体mRNA的表达增强有关。
