Joaquin M, Rosa J L, Bartrons R, Tauler A
Departament de Cièncias Fisiòlogiques Humanes i de la Nutrició, Universitat de Barcelona, Catalunya, Spain.
Biochim Biophys Acta. 1997 Mar 15;1334(2-3):256-60. doi: 10.1016/s0304-4165(96)00105-5.
The expression of the F-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA was studied during liver regeneration by three independent assays: Northern blot analysis, reverse transcription-polymerase chain reaction and ribonuclease protection. We demonstrate the presence of F-type mRNA in foetal and adult rat livers and a transient increase in its levels with a maximum at 12 h after partial liver resection. The time course of F-type mRNA induction differs from that reported for the L-type isoform, suggesting differences in the regulation of the expression of F- and L-type isoforms of the bifunctional enzyme during liver regeneration.
通过三种独立检测方法（Northern印迹分析、逆转录-聚合酶链反应和核糖核酸酶保护法）对肝脏再生过程中F型6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶mRNA的表达进行了研究。我们证明在胎鼠和成年大鼠肝脏中存在F型mRNA，并且在部分肝切除术后12小时其水平出现短暂升高并达到最大值。F型mRNA诱导的时间进程与报道的L型同工型不同，这表明在肝脏再生过程中双功能酶的F型和L型同工型的表达调控存在差异。
