Oliunina L N, Shabaev V P
Mikrobiologiia. 1996 Nov-Dec;65(6):813-7.
The production of indole-3-acetic acid (IAA) by batch cultures of the rhizosphere bacteria Pseudomonas fluorescens 20 and Pseudomonas putida 23, known to stimulate plant growth, was studied by immunoenzymatic analysis and the Gordon-Weber method. The accumulation of IAA exhibited two maxima, in the 9th and 40th hours of cultivation. In both pseudomonad cultures, the first maximum occurred in the exponential phase, and the second maximum corresponded to the beginning of the stationary growth phase. Both maxima of IAA accumulation coincided with decreases in the specific growth rates of the cultures. The stationary-phase culture of P. putida 23, as compared to that of P. fluorescens 20, was characterized by greater IAA content, which was retained for a long time.
已知能促进植物生长的根际细菌荧光假单胞菌20和恶臭假单胞菌23的分批培养物中吲哚-3-乙酸（IAA）的产生，通过免疫酶分析和戈登-韦伯方法进行了研究。IAA的积累呈现出两个最大值，分别出现在培养的第9小时和第40小时。在两种假单胞菌培养物中，第一个最大值出现在指数期，第二个最大值对应于稳定生长阶段的开始。IAA积累的两个最大值都与培养物比生长速率的下降同时出现。与荧光假单胞菌20相比，恶臭假单胞菌23的稳定期培养物的特点是IAA含量更高，且能长时间保持。
