Stingl J, Kos J V
Department of Normal Anatomy, Medical Faculty, Karlov University, Plzen, CSSR.
Arkh Anat Gistol Embriol. 1981 Dec;81(12):72-81.
An original simple method was successfully worked out when following systematically the ultrastructure of particular microcirculatory areas of the m. spinotrapezius in the white rat. The main principle of this method consists in a precise orientation of the selected parts of the terminal vascular bed, beginning from their vital observation, continuing through their fixation and dehydration and ending with their embedding into a thin layer of Epon in order to prepare blocks containing very small of the beforehand-chosen areas of the vessels studied. A concise survey of the results obtained is given with a description of the main structural elements specific for the wall of the arterioles, terminal and precapillary arterioles, capillaries, postcapillaries and larger venules. The method presented has proved to be practically useful and is recommended for further investigations concerned with the submicroscopical structure of muscular vessels.
在对白鼠斜方肌特定微循环区域的超微结构进行系统追踪时，成功研发出一种新颖简便的方法。该方法的主要原理在于，从对终末血管床选定部分的活体观察开始，经固定、脱水处理，直至将其包埋于一层薄薄的环氧树脂中，从而制备出包含极少量预先选定研究血管区域的组织块，实现对终末血管床选定部分的精确取向。文中给出了所得结果的简要概述，并描述了小动脉、终末小动脉和毛细血管前小动脉、毛细血管、毛细血管后小静脉及较大静脉壁特有的主要结构元件。所提出的方法已证明具有实际应用价值，推荐用于进一步研究肌肉血管的亚微观结构。
