Cox T L, Sly L I
Department of Microbiology, University of Queensland, Brisbane, Australia.
Int J Syst Bacteriol. 1997 Apr;47(2):377-80. doi: 10.1099/00207713-47-2-377.
The phylogenetic relationships among the species of the genus Pedomicrobium were studied by comparing their 16S rRNA sequences. The Pedomicrobium species form a coherent phylogenetic cluster within the genera of the hyphal budding bacteria in the alpha-Proteobacteria. The sequences of two strains of Pedomicrobium australicum were obtained from DNAs extracted from nonviable freeze-dried cells, which are the only source of material available, and were found to be almost identical (level of similarity, 99.9%). Overall, the Pedomicrobium species are closely related, with sequence similarities ranging from 96.2 to 99.9%. Pedomicrobium manganicum is phylogenetically the most distantly related species and exhibits the lowest similarity (96.2%) with Pedomicrobium americanum. Australian isolate Pedomicrobium sp. strain ACM 3067, P. americanum, and P. australicum are all very highly related, with similarities greater than 99%. Pedomicrobium sp. strain ACM 3067 is most closely related to P. australicum (level of similarity, 99.6%) and P. americanum (99.4%). These manganese-oxidizing species are more closely related to the iron-oxidizing species Pedomicrobium ferrugineum than to the other manganese-oxidizing species, P. manganicum. Taxonomic uncertainties resulting from the loss of the type culture of P. australicum are discussed.
通过比较嗜微菌属物种的16S rRNA序列，研究了它们之间的系统发育关系。嗜微菌属物种在α-变形菌纲的菌丝出芽细菌属中形成了一个连贯的系统发育簇。两株澳大利亚嗜微菌的序列是从非活性冻干细胞中提取的DNA获得的，这是唯一可用的材料来源，发现它们几乎相同（相似性水平为99.9%）。总体而言，嗜微菌属物种密切相关，序列相似性范围为96.2%至99.9%。锰嗜微菌在系统发育上是关系最远的物种，与美洲嗜微菌的相似性最低（96.2%）。澳大利亚分离株嗜微菌属菌株ACM 3067、美洲嗜微菌和澳大利亚嗜微菌都高度相关，相似性大于99%。嗜微菌属菌株ACM 3067与澳大利亚嗜微菌（相似性水平为99.6%）和美洲嗜微菌（99.4%）关系最密切。这些锰氧化物种与铁氧化物种铁锈嗜微菌的关系比与其他锰氧化物种锰嗜微菌的关系更密切。讨论了由于澳大利亚嗜微菌模式培养物丢失导致的分类学不确定性。
