Kato A
Department of Otolaryngology, Yamada Red Cross Hospital, Mie.
Nihon Jibiinkoka Gakkai Kaiho. 1997 Mar;100(3):351-6. doi: 10.3950/jibiinkoka.100.351.
Since erythromycin was shown to be effective in the treatment of patients with diffuse panbronchiolitits, newly discovered effects of macrolide antibiotics have attracted much attention. It was reported that erythromycin inhibits Cl secretion across cultured canine tracheal epithelial cells. Erythromycin may decrease the movement of water toward the lumen, thus reducing sputum volume. We tested the hypothesis that erythromycin and clarithromycin have a similar effect on the dissected canine tracheal epithelium, by measuring the short circuit current using Ussing chambers. Addition of erythromycin or clarithromycin did not change the short circuit current within 20 minutes when applied on either the mucosal side or the submucosal side. No changes in the short circuit current were observed after pretreatment of the epithelium with amiloride, an Na channel blocker, or bumetanide, a Cl transport inhibitor, and subsequent addition of the macrolide antibiotics. These data indicate that neither erythromycin nor clarithromycin has any short term effect on ion transport in the dissected canine tracheal epithelium.
自从红霉素被证明对弥漫性泛细支气管炎患者有效以来，大环内酯类抗生素新发现的作用引起了广泛关注。据报道，红霉素可抑制培养的犬气管上皮细胞的氯离子分泌。红霉素可能会减少水分向管腔的移动，从而减少痰液量。我们通过使用Ussing小室测量短路电流，来检验红霉素和克拉霉素对分离的犬气管上皮具有相似作用这一假设。当将红霉素或克拉霉素应用于黏膜侧或黏膜下层时，在20分钟内短路电流没有变化。在用钠通道阻滞剂氨氯吡咪或氯转运抑制剂布美他尼对上皮进行预处理，随后添加大环内酯类抗生素后，未观察到短路电流有变化。这些数据表明，红霉素和克拉霉素对分离的犬气管上皮中的离子转运均无短期作用。
