Tsanev R, Yaneva J, Vaptzarova K, Markov D
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Acta Biochim Pol. 1996;43(4):593-601.
A dosed mechanical pressure of 12.5 kg/cm2 applied for 1 min on depilated mouse skin did not cause cellular death or visible alterations of the cellular ultrastructures. However, it had a strong effect on the mitotic cycle of the epidermal cells-stimulating the cells to enter the mitotic cycle and temporarily blocking the G1-->S transition. This effect was strictly limited to the pressed area of the skin. The proto-oncogene c-fos was induced within the first 2 min following application of the pressure. The level of c-fos mRNA showed two peaks during the next 24 h. The first slight peak was preceded by a rapid increase in the cAMP level in the pressed skin, the second-by a fall in the cAMP concentration. A model is suggested to explain the observed effects by reversible functional damage of the cellular membrane affecting the enzymes maintaining the steady state level of cAMP.
对脱毛后的小鼠皮肤施加12.5千克/平方厘米的定量机械压力1分钟，并未导致细胞死亡或细胞超微结构出现可见改变。然而，它对表皮细胞的有丝分裂周期有强烈影响——刺激细胞进入有丝分裂周期并暂时阻断G1期向S期的转变。这种效应严格局限于皮肤的受压区域。原癌基因c-fos在施加压力后的最初2分钟内被诱导。在接下来的24小时内，c-fos mRNA水平出现两个峰值。第一个轻微峰值之前，受压皮肤中的cAMP水平迅速升高，第二个峰值之前，cAMP浓度下降。有人提出一个模型，通过影响维持cAMP稳态水平的酶的细胞膜可逆性功能损伤来解释观察到的效应。
