Belke D D, Wang L C, Lopaschuk G D
Department of Pharmacology, University of Alberta, Edmonton, Canada.
J Appl Physiol (1985). 1997 Apr;82(4):1210-8. doi: 10.1152/jappl.1997.82.4.1210.
Glycolysis, glucose oxidation, palmitate oxidation, and cardiac function were measured in isolated working hearts from ground squirrels and rats subjected to a hypothermia-rewarming protocol. Hearts were perfused initially for 30 min at 37 degrees C, followed by 2 h of hypothermic perfusion at 15 degrees C, after which hearts were rewarmed to 37 degrees C and further perfused for 30 min. Functional recovery in ground squirrel hearts was greater than in rat hearts after rewarming. Hypothermia-rewarming had a similar general effect on the various metabolic pathways in both species. Despite these similarities, total energy substrate metabolic rates were greater in rat than ground squirrel hearts during hypothermia despite a lower level of work being performed by the rat hearts, indicating that rat hearts are less efficient than ground squirrel hearts during hypothermia. After rewarming, energy substrate metabolism recovered completely in both species, although cardiac work remained depressed in rat hearts. The difference in functional recovery between rat and ground squirrel hearts after rewarming cannot be explained by general differences in energy substrate metabolism during hypothermia or after rewarming.
在经历了低温复温过程的地松鼠和大鼠的离体工作心脏中，对糖酵解、葡萄糖氧化、棕榈酸氧化和心脏功能进行了测量。心脏最初在37℃灌注30分钟，随后在15℃进行2小时的低温灌注，之后将心脏复温至37℃并进一步灌注30分钟。复温后，地松鼠心脏的功能恢复比大鼠心脏更好。低温复温对两个物种的各种代谢途径具有相似的总体影响。尽管存在这些相似之处，但在低温期间，大鼠心脏的总能量底物代谢率高于地松鼠心脏，尽管大鼠心脏的工作量较低，这表明在低温期间大鼠心脏的效率低于地松鼠心脏。复温后，两个物种的能量底物代谢均完全恢复，尽管大鼠心脏的心脏工作仍受到抑制。大鼠和地松鼠心脏复温后功能恢复的差异不能用低温期间或复温后能量底物代谢的一般差异来解释。
