Harnett W, MacDonald M, Preece G, Patterson M, Parkhouse R M
Department of Immunology, University of Strathclyde, Glasgow, U.K.
J Parasitol. 1997 Apr;83(2):316-9.
Nine monoclonal antibodies (mabs) have been produced against excretory-secretory products (ES) of adult male Onchocerca gibsoni. These mabs fail to interact with the highly cross-reactive phosphorylcholine (PC) group and with ES of the related rodent filarial parasites Acanthocheilonema viteae and Litomosoides carinii. Eight of the mabs are of the IgG isotype: 1 is an IgM. Three of the mabs, OGMES 4, 9, and 10, were each found by immunoprecipitation/SDS-PAGE analysis of [3H] leucine-labeled ES, to recognize a triplet of polypeptides of molecular weight 120, approximately 210, and approximately 260 kDa. No recognition was observed by any mab when [3H] glucosamine was employed as the radiolabel for ES. Western blotting employing [125I] as indicator system demonstrated that OGMES 7 recognized a molecule of 27 kDa, and OGMES 1, a molecule of approximately 210 kDa, albeit faintly. These mabs may be of value to researchers working on the isolation, characterization, and detection in the bloodstream of Onchocerca volvulus ES.
已制备出9种针对成年雄性吉氏盘尾丝虫排泄分泌产物（ES）的单克隆抗体（mabs）。这些单克隆抗体不与高度交叉反应的磷酸胆碱（PC）基团以及相关啮齿类丝状寄生虫棘唇旋盘尾丝虫和卡里尼丝虫的ES相互作用。其中8种单克隆抗体为IgG同种型，1种为IgM。通过对[3H]亮氨酸标记的ES进行免疫沉淀/SDS-PAGE分析发现，3种单克隆抗体OGMES 4、9和10各自识别一组分子量分别约为120、210和260 kDa的三条多肽。当使用[3H]葡糖胺作为ES的放射性标记时，未观察到任何单克隆抗体的识别反应。采用[125I]作为指示系统的蛋白质印迹法表明，OGMES 7识别一个27 kDa的分子，OGMES 1识别一个约210 kDa的分子，不过识别较弱。这些单克隆抗体可能对致力于盘尾丝虫ES在血液中的分离、表征和检测的研究人员有价值。
