Hoshi K, Amizuka N, Oda K, Ikehara Y, Ozawa H
1st Department of Oral Anatomy, Niigata University School of Dentistry, Japan.
Histochem Cell Biol. 1997 Mar;107(3):183-91. doi: 10.1007/s004180050103.
Immunolocalization of tissue non-specific alkaline phosphatase (TNAP) was examined in murine tissues, employing a specific antiserum to TNAP on frozen sections, 50-micron tissue slices, and paraffin sections. TNAP was detected at high levels in hard tissues including bone, cartilage, and tooth. In bone tissue, the TNAP immunoreactivity was localized on the entire cell surface of preosteoblasts, as well as the basolateral cell membrane of osteoblasts. It was also localized on some resting chondrocytes and most of the proliferative and hypertrophic cells in cartilage. In the incisor, cells of the stratum intermedium, the subodontoblastic layer, the proximal portion of secretory ameloblasts, and the basolateral portion of odontoblasts showed particularly strong immunoreactivity. Immunoreactivity was observed in other soft tissues, such as the brush borders of proximal renal tubules in kidney, on cell membrane of the biliary canalicula in liver and in trophoblasts in the placenta. These immunolocalizations were quite similar to enzyme histochemical localizations. However, neither the submandibular gland nor the intestine, which both exhibited alkaline phosphatase activity by enzyme histochemistry, revealed immunoreactivity for TNAP. Therefore, immunocytohistochemical studies for TNAP enabled us to localize the TNAP isozyme, thus distinguishing it from other isozymes.
利用针对组织非特异性碱性磷酸酶（TNAP）的特异性抗血清，在小鼠组织的冰冻切片、50微米厚的组织切片和石蜡切片上检测TNAP的免疫定位。在包括骨、软骨和牙齿在内的硬组织中检测到高水平的TNAP。在骨组织中，TNAP免疫反应性定位于前成骨细胞的整个细胞表面以及成骨细胞的基底外侧细胞膜。它也定位于软骨中的一些静止软骨细胞以及大多数增殖和肥大细胞。在切牙中，中间层细胞、成牙本质细胞下层、分泌性成釉细胞的近端部分以及成牙本质细胞的基底外侧部分显示出特别强的免疫反应性。在其他软组织中也观察到免疫反应性，如肾近端小管的刷状缘、肝胆小管的细胞膜以及胎盘的滋养层细胞。这些免疫定位与酶组织化学定位非常相似。然而，通过酶组织化学显示具有碱性磷酸酶活性的下颌下腺和肠均未显示出TNAP的免疫反应性。因此，对TNAP的免疫细胞组织化学研究使我们能够定位TNAP同工酶，从而将其与其他同工酶区分开来。
