采用等度高效液相色谱法测定可溶性鸟苷酸环化酶活性。

Assay of soluble guanylate cyclase activity by isocratic high-performance liquid chromatography.

作者信息

Pietta P G, Mauri P L, Gardana C, Benazzi L

机构信息

ITBA-CNR, Milan, Italy.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Mar 7;690(1-2):343-7. doi: 10.1016/s0378-4347(96)00414-8.

DOI:10.1016/s0378-4347(96)00414-8

PMID:9106063

Abstract

A HPLC method alternative to labelled or unlabelled procedures was developed for the assay of guanylate cyclase (GC) activity. The substrate (GTP) and the product (cGMP) of the enzymatic reaction were separated in the isocratic mode on a muBondapak C18 column. The activity of GC was linearly dependent on the amount of cGMP produced in the presence of sodium nitroprusside. This approach was applied to follow the purification of GC from bovine lung and to evaluate its stability in different storage conditions.

摘要

开发了一种用于测定鸟苷酸环化酶（GC）活性的HPLC方法，该方法可替代标记或未标记的程序。酶促反应的底物（GTP）和产物（cGMP）在muBondapak C18柱上以等度洗脱模式分离。在硝普钠存在下，GC的活性与产生的cGMP量呈线性相关。该方法用于跟踪从牛肺中纯化GC的过程，并评估其在不同储存条件下的稳定性。