Guihot G, Merle V, Leborgne M, Pivert G, Corriol O, Brousse N, Ricour C, Colomb V
Unité d'Ecologie et de Physiologie du Système Digestif, Jouy-En-Josas, France.
J Pediatr Gastroenterol Nutr. 1997 Feb;24(2):153-61. doi: 10.1097/00005176-199702000-00008.
It has been suggested that beneficial effect of elemental enteral diets in the treatment of inflammatory bowel diseases could be mediated by the suppression of protein dietary antigens. The objective of the present work was to study the effect of enteral diet on gut associated lymphoid tissue and on gastric Lactobacillus flora, in rat.
The effects of three molecular forms of nitrogen supply: amino acids, oligopeptides or whole casein, were compared in rats on continuous enteral diet. Frozen sections of small bowel were studied with monoclonal antibodies anti-CD5, -CD4, -CD8, -CD25, -macrophages, -MHC II. The Lactobacillus flora was also enumerated in the stomach, in order to assess the effect of ED on rat flora.
Growth and mucosa morphology were identical in control and enteral groups. Rats on enteral diet showed, whatever was the molecular form of nitrogen supply, a decrease in CD5+, CD4+ and CD8+ intraepithelial cell numbers, but not in lamina propria cell number, and a decreased MHC II epithelial expression, when compared to control rats. The enterally fed rats also showed a decrease in Lactobacillus gastric contents.
The current study demonstrates that continuous enteral nutrition modifies MHC II epithelial expression and gut associated lymphoid tissue cell number in rat, whatever is the molecular form of nitrogen supply. Intestinal flora could be responsible, at least for part, for these results.
有人提出，要素肠内营养饮食在治疗炎症性肠病中的有益作用可能是通过抑制蛋白质饮食抗原介导的。本研究的目的是探讨肠内营养饮食对大鼠肠道相关淋巴组织及胃内乳酸杆菌菌群的影响。
比较了三种分子形式的氮源供应（氨基酸、寡肽或全酪蛋白）对持续接受肠内营养饮食的大鼠的影响。用抗CD5、-CD4、-CD8、-CD25、巨噬细胞、MHC II的单克隆抗体研究小肠冰冻切片。还对胃内的乳酸杆菌菌群进行了计数，以评估肠内营养饮食对大鼠菌群的影响。
对照组和肠内营养组的生长和黏膜形态相同。与对照大鼠相比，接受肠内营养饮食的大鼠，无论氮源供应的分子形式如何，其CD5 +、CD4 +和CD8 +上皮内细胞数量均减少，但固有层细胞数量未减少，且MHC II上皮表达降低。接受肠内营养喂养的大鼠胃内容物中的乳酸杆菌数量也减少。
当前研究表明，无论氮源供应的分子形式如何，持续肠内营养都会改变大鼠的MHC II上皮表达和肠道相关淋巴组织细胞数量。肠道菌群可能至少部分地导致了这些结果。
