Holappa L D, Walker-Simmons M K
U.S. Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research, Washington State University, Pullman 99164-6420, USA.
Plant Mol Biol. 1997 Mar;33(5):935-41. doi: 10.1023/a:1005720203535.
We have identified a new wheat PKABA1-like protein kinase gene, TaPK3, that is expressed in greening wheat seedlings. TaPK3 has high sequence homology (97% similarity with some sequence diversity at the 3' end) to the wheat PKABA1 protein kinase mRNA, which is upregulated by cold-temperature treatment, dehydration and abscisic acid (ABA). Use of a TaPK3 gene-specific probe has revealed that TaPK3 is differentially expressed with respect to PKABA1. TaPK3 mRNA accumulates in greening shoot tissue of wheat, but is not affected by dehydration, cold-temperature treatment or ABA. Based on sequence and expression differences, we conclude that expression of the PKABA1-like protein kinases is not limited to stress responses.
我们鉴定出一个新的小麦类PKABA1蛋白激酶基因TaPK3，它在小麦绿化幼苗中表达。TaPK3与小麦PKABA1蛋白激酶mRNA具有高度的序列同源性（97%相似，3'端存在一些序列差异），后者在低温处理、脱水和脱落酸（ABA）作用下上调表达。使用TaPK3基因特异性探针显示，TaPK3与PKABA1的表达存在差异。TaPK3 mRNA在小麦绿化的地上组织中积累，但不受脱水、低温处理或ABA的影响。基于序列和表达差异，我们得出结论，类PKABA1蛋白激酶的表达不限于应激反应。
