vom Dahl S, Häussinger D
Klinik für Gastroenterologie, Hepatologie und Infektiologie, Medizinische Einrichtangen der Heinrich-Heine Universität, Düsseldorf.
Z Gastroenterol. 1997 Mar;35(3):221-6.
Perfused rat liver is a well-established model for studies on hepatic metabolism. The different perfusion systems, the technical requirements and the surgical preparation steps are described. A main advantage of liver perfusion is the maintenance of liver architecture-rendering it a feasible model for the study of interactions between parenchymal and non-parenchymal cells. Furthermore, steady-state conditions allow the calculation of metabolic flux rates and the reversibility of agonist-induced effects can be studied within the same preparation. As the polarity of the cells is maintained, sinusoidal uptake, metabolism and biliary excretion of substances can be studied. Some applications of liver perfusion and special techniques are described, the advantages specified and the limitations of this model discussed. Due to recent developments in monitoring of liver hemodynamics, extracellular ion concentrations and changes of liver cell volume, liver perfusion is one of the best-controlled experimental systems in the study of hepatic physiology.
灌注大鼠肝脏是研究肝脏代谢的一个成熟模型。本文描述了不同的灌注系统、技术要求和手术准备步骤。肝脏灌注的一个主要优点是维持肝脏结构，使其成为研究实质细胞和非实质细胞之间相互作用的可行模型。此外，稳态条件允许计算代谢通量率，并且可以在同一制剂中研究激动剂诱导效应的可逆性。由于细胞极性得以维持，因此可以研究物质的窦状隙摄取、代谢和胆汁排泄。本文描述了肝脏灌注的一些应用和特殊技术，明确了其优点并讨论了该模型的局限性。由于最近在肝脏血流动力学监测、细胞外离子浓度和肝细胞体积变化监测方面的进展，肝脏灌注是肝脏生理学研究中控制最好的实验系统之一。
