Webster M, Libranda-Ramirez B D, Aligui G D, Olveda R M, Ouma J H, Kariuki H C, Kimani G, Olds G R, Fulford A J, Butterworth A E, Dunne D W
Department of Pathology, University of Cambridge.
Parasitology. 1997 Apr;114 ( Pt 4):383-93. doi: 10.1017/s003118209600858x.
We have investigated the effects of host age and sex on human antibody isotype responses to Schistosoma mansoni and Schistosoma japonicum adult worm (AW) and soluble egg (SEA) antigens, using sera from subjects in Kenya and the Philippines. Similar trends with age were observed between the two populations despite host, parasite and environmental differences between the two geographical locations. IgE to AW increased with age, whereas most isotype responses to SEA decreased with age. IgG1, IgG3 and IgG4 subclass responses to adult worm, however, did not show a broadly rising or falling pattern with age. Males were found to have higher IgG1, IgG4 and IgE to AW in both populations. This sex difference remained significant in the Kenyan population even after controlling statistically for confounding factors such as age and differences in intensity of infection. Analysis of S. mansoni and S. japonicum adult worm antigens reactive with IgE revealed a predominant 22 kDa band in both parasites. Only those individuals with relatively high IgE titres specifically reactive with S. mansoni or S. japonicum AW had detectable IgE against Sj22 or Sm22.
我们利用肯尼亚和菲律宾受试者的血清，研究了宿主年龄和性别对人体针对曼氏血吸虫和日本血吸虫成虫（AW）及可溶性虫卵（SEA）抗原的抗体同种型反应的影响。尽管两个地理位置的宿主、寄生虫和环境存在差异，但在这两个人群中观察到了与年龄相关的相似趋势。针对AW的IgE随年龄增加，而针对SEA的大多数同种型反应随年龄降低。然而，针对成虫的IgG1、IgG3和IgG4亚类反应并未呈现出随年龄普遍上升或下降的模式。在两个人群中，男性针对AW的IgG1、IgG4和IgE水平均较高。即使在对年龄和感染强度差异等混杂因素进行统计学控制后，肯尼亚人群中的这种性别差异仍然显著。对与IgE反应的曼氏血吸虫和日本血吸虫成虫抗原的分析显示，两种寄生虫中均有一条主要的22 kDa条带。只有那些IgE滴度相对较高且与曼氏血吸虫或日本血吸虫AW特异性反应的个体，才具有针对Sj22或Sm22的可检测IgE。
