嗜酸性红藻嗜热栖热放线菌质膜H(+) -ATP酶编码基因的克隆与测序

Cloning and sequencing of the gene encoding the plasma membrane H(+)-ATPase from an acidophilic red alga, Cyanidium caldarium.

作者信息

Ohta H, Shirakawa H, Uchida K, Yoshida M, Matuo Y, Enami I

机构信息

Department of Biology, Faculty of Science, Science University of Tokyo, Japan.

出版信息

Biochim Biophys Acta. 1997 Mar 28;1319(1):9-13. doi: 10.1016/s0005-2728(97)00007-8.

DOI:10.1016/s0005-2728(97)00007-8

PMID:9107313

Abstract

A cDNA containing an open reading frame encoding the putative plasma membrane H(+)-ATPase in an acidophilic red alga, Cyanidium caldarium, was cloned and sequenced by means of PCR and Southern hybridization based on homologous sequences of P-type ATPases found in other organisms. The cloned cDNA is 3300 bp in length, containing a 2865 bp open reading frame encoding a polypeptide of 955 amino acids which has a predicted molecular mass of 105,371. The deduced amino acid sequence was found to be more homologous to those of P-type H(+)-ATPases from higher plants than that from the green alga Dunaliella bioculata.

摘要

通过基于其他生物中发现的P型ATP酶的同源序列进行PCR和Southern杂交，克隆并测序了一个来自嗜酸性红藻蓝纤维藻（Cyanidium caldarium）的cDNA，该cDNA含有一个编码假定质膜H(+) -ATP酶的开放阅读框。克隆的cDNA长度为3300 bp，包含一个2865 bp的开放阅读框，编码一个由955个氨基酸组成的多肽，预测分子量为105,371。发现推导的氨基酸序列与高等植物的P型H(+) -ATP酶的氨基酸序列比与绿藻杜氏盐藻（Dunaliella bioculata）的P型H(+) -ATP酶的氨基酸序列更同源。