培养物中果蝇周期基因表达的节律
Rhythms of Drosophila period gene expression in culture.
作者信息
Emery I F, Noveral J M, Jamison C F, Siwicki K K
机构信息
Department of Biology, Swarthmore College, PA 19081, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):4092-6. doi: 10.1073/pnas.94.8.4092.
Abstract
The Drosophila clock genes period (per) and timeless (tim) have been studied behaviorally and biochemically, but to date there has been no viable culture system for studying the cell biology of the Drosophila clock. We have cultured pupal ring glands attached to the central nervous system and observed rhythms of period gene expression in the prothoracic gland for 4-7 days. A daily rhythm of Per protein can be entrained by light in culture, even when neural activity is blocked by tetrodotoxin. In cultures maintained for a week in constant darkness, a per-luciferase reporter gene revealed circadian rhythms of bioluminescence. As the first circadian culture system from Drosophila, the prothoracic gland provides unique advantages for investigating the interactions between clock genes and cellular physiology.
摘要
果蝇生物钟基因周期基因（per）和无时间基因（tim）已通过行为学和生物化学方法进行了研究，但迄今为止，还没有可行的培养系统来研究果蝇生物钟的细胞生物学。我们培养了附着于中枢神经系统的蛹期环腺，并观察前胸腺中周期基因表达的节律达4至7天。即使神经活动被河豚毒素阻断，培养物中的Per蛋白每日节律仍可被光诱导。在持续黑暗中维持一周的培养物中，一个per - 荧光素酶报告基因显示出生物发光的昼夜节律。作为首个来自果蝇的昼夜节律培养系统，前胸腺为研究生物钟基因与细胞生理学之间的相互作用提供了独特优势。
相似文献
1
Rhythms of Drosophila period gene expression in culture.培养物中果蝇周期基因表达的节律
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):4092-6. doi: 10.1073/pnas.94.8.4092.
2
Disruption of synaptic transmission or clock-gene-product oscillations in circadian pacemaker cells of Drosophila cause abnormal behavioral rhythms.果蝇昼夜节律起搏器细胞中突触传递的破坏或生物钟基因产物的振荡会导致异常的行为节律。
J Neurobiol. 2000 Jun 5;43(3):207-33. doi: 10.1002/(sici)1097-4695(20000605)43:3<207::aid-neu1>3.0.co;2-0.
3
double-time is a novel Drosophila clock gene that regulates PERIOD protein accumulation.双时基因是一种新型果蝇时钟基因，可调节周期蛋白的积累。
Cell. 1998 Jul 10;94(1):83-95. doi: 10.1016/s0092-8674(00)81224-6.
4
Rhythmic expression of timeless: a basis for promoting circadian cycles in period gene autoregulation.“永恒”基因的节律性表达：促进周期基因自动调节中昼夜节律循环的基础。
Science. 1995 Nov 3;270(5237):808-10. doi: 10.1126/science.270.5237.808.
5
Molecular and phylogenetic analyses reveal mammalian-like clockwork in the honey bee (Apis mellifera) and shed new light on the molecular evolution of the circadian clock.分子和系统发育分析揭示了蜜蜂（西方蜜蜂）中类似哺乳动物的生物钟机制，并为昼夜节律钟的分子进化提供了新线索。
Genome Res. 2006 Nov;16(11):1352-65. doi: 10.1101/gr.5094806. Epub 2006 Oct 25.
6
Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands.神经元对蛹期果蝇前胸腺外周昼夜节律振荡器的影响。
Nat Commun. 2012 Jun 19;3:909. doi: 10.1038/ncomms1922.
7
Differential effects of light and heat on the Drosophila circadian clock proteins PER and TIM.光和热对果蝇生物钟蛋白PER和TIM的不同影响。
Mol Cell Biol. 1998 Apr;18(4):2004-13. doi: 10.1128/MCB.18.4.2004.
8
Suppression of PERIOD protein abundance and circadian cycling by the Drosophila clock mutation timeless.果蝇生物钟突变体timeless对周期蛋白丰度和昼夜节律循环的抑制作用。
EMBO J. 1995 Aug 15;14(16):4044-9. doi: 10.1002/j.1460-2075.1995.tb00075.x.
9
The F-box protein slimb controls the levels of clock proteins period and timeless.F-box蛋白slimb控制生物钟蛋白周期蛋白（period）和无时间蛋白（timeless）的水平。
Nature. 2002 Nov 14;420(6912):178-82. doi: 10.1038/nature01122.
10
Modeling the molecular regulatory mechanism of circadian rhythms in Drosophila.模拟果蝇昼夜节律的分子调控机制。
Bioessays. 2000 Jan;22(1):84-93. doi: 10.1002/(SICI)1521-1878(200001)22:1<84::AID-BIES13>3.0.CO;2-I.
引用本文的文献
1
Fluorescent Reporters for Studying Circadian Rhythms in Drosophila melanogaster.用于研究黑腹果蝇昼夜节律的荧光报告基因。
Methods Mol Biol. 2022;2482:353-371. doi: 10.1007/978-1-0716-2249-0_24.
2
Only time will tell: the interplay between circadian clock and metabolism.唯有时间能证明一切：生物钟与新陈代谢的相互作用。
Chronobiol Int. 2021 Feb;38(2):149-167. doi: 10.1080/07420528.2020.1842436. Epub 2020 Dec 20.
3
Reflections on contributing to "big discoveries" about the fly clock: Our fortunate paths as post-docs with 2017 Nobel laureates Jeff Hall, Michael Rosbash, and Mike Young.关于为果蝇生物钟“重大发现”做出贡献的思考：我们作为博士后与2017年诺贝尔奖获得者杰夫·霍尔、迈克尔·罗斯巴什和迈克·扬的幸运历程。
Neurobiol Sleep Circadian Rhythms. 2018 Mar 16;5:58-67. doi: 10.1016/j.nbscr.2018.02.004. eCollection 2018 Jun.
4
Nighttime activities and peripheral clock oscillations depend on Wolbachia endosymbionts in flies.夜间活动和外周时钟振荡依赖于果蝇中的沃尔巴克氏体共生菌。
Sci Rep. 2018 Oct 18;8(1):15432. doi: 10.1038/s41598-018-33522-8.
5
Central and peripheral clocks are coupled by a neuropeptide pathway in Drosophila.果蝇的神经肽途径将中枢时钟和外周时钟耦合。
Nat Commun. 2017 May 30;8:15563. doi: 10.1038/ncomms15563.
6
Heterogeneity of the Peripheral Circadian Systems in Drosophila melanogaster: A Review.黑腹果蝇外周昼夜节律系统的异质性：综述
Front Physiol. 2016 Jan 29;7:8. doi: 10.3389/fphys.2016.00008. eCollection 2016.
7
Circadian Clock Genes Modulate Human Bone Marrow Mesenchymal Stem Cell Differentiation, Migration and Cell Cycle.昼夜节律时钟基因调节人骨髓间充质干细胞的分化、迁移和细胞周期。
PLoS One. 2016 Jan 7;11(1):e0146674. doi: 10.1371/journal.pone.0146674. eCollection 2016.
8
Developmental and light-entrained expression of melatonin and its relationship to the circadian clock in the sea anemone Nematostella vectensis.发育和光照对海葵 Nematostella vectensis 中褪黑素的表达及其与生物钟的关系。
Evodevo. 2014 Aug 14;5:26. doi: 10.1186/2041-9139-5-26. eCollection 2014.
9
Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands.神经元对蛹期果蝇前胸腺外周昼夜节律振荡器的影响。
Nat Commun. 2012 Jun 19;3:909. doi: 10.1038/ncomms1922.
10
Cellular requirements for LARK in the Drosophila circadian system.果蝇生物钟系统中 LARK 的细胞需求。
J Biol Rhythms. 2012 Jun;27(3):183-95. doi: 10.1177/0748730412440667.
本文引用的文献
1
Localization of a circadian pacemaker in the eye of a mollusc, bulla.在软体动物的眼睛——笠贝中定位生物钟的位置。
Science. 1982 Jul 9;217(4555):155-7. doi: 10.1126/science.217.4555.155.
2
Prothoracic glands of the saturniid moth Samia cynthia ricini possess a circadian clock controlling gut purge timing.Saturniid moth Samia cynthia ricini 的前胸腺具有控制肠道排空时间的生物钟。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2726-30. doi: 10.1073/pnas.79.8.2726.
3
Regulation of nuclear entry of the Drosophila clock proteins period and timeless.果蝇生物钟蛋白周期蛋白（period）和无时间蛋白（timeless）进入细胞核的调控
Neuron. 1996 Nov;17(5):911-20. doi: 10.1016/s0896-6273(00)80222-6.
4
Regulation of the Drosophila protein timeless suggests a mechanism for resetting the circadian clock by light.果蝇中“无时间”蛋白的调控揭示了一种通过光重置生物钟的机制。
Cell. 1996 Mar 8;84(5):677-85. doi: 10.1016/s0092-8674(00)81046-6.
5
Novel features of drosophila period Transcription revealed by real-time luciferase reporting.实时荧光素酶报告揭示的果蝇周期转录新特征。
Neuron. 1996 Apr;16(4):687-92. doi: 10.1016/s0896-6273(00)80088-4.
6
Circadian rhythms in cultured mammalian retina.培养的哺乳动物视网膜中的昼夜节律。
Science. 1996 Apr 19;272(5260):419-21. doi: 10.1126/science.272.5260.419.
7
A light-entrainment mechanism for the Drosophila circadian clock.果蝇生物钟的光诱导机制。
Nature. 1996 Mar 14;380(6570):129-35. doi: 10.1038/380129a0.
8
Resetting the Drosophila clock by photic regulation of PER and a PER-TIM complex.通过对PER和PER-TIM复合物的光调节来重置果蝇生物钟。
Science. 1996 Mar 22;271(5256):1740-4. doi: 10.1126/science.271.5256.1740.
9
Light-induced degradation of TIMELESS and entrainment of the Drosophila circadian clock.光诱导的TIMELESS降解与果蝇生物钟的同步化
Science. 1996 Mar 22;271(5256):1736-40. doi: 10.1126/science.271.5256.1736.
10
Circadian clock functions localized in xenopus retinal photoreceptors.生物钟功能定位于非洲爪蟾视网膜光感受器中。
Neuron. 1993 Apr;10(4):573-7. doi: 10.1016/0896-6273(93)90160-s.
Zotero 插件