Drénou B, Lamy T, Amiot L, Fardel O, Caulet-Maugendre S, Sasportes M, Diebold J, Le Prisé P Y, Fauchet R
Laboratoire d'Hématologie-Immunologie, Hôpital Pontchaillou, CHRU Rennes, France.
Blood. 1997 Apr 15;89(8):2966-74.
CD56 expression has been reported previously in some non-Hodgkin's lymphoma (NHL) characterization. They principally involve the nasopharynx, are related to Epstein-Barr virus (EBV), and may be classified as either T- or non-T-natural killer (NK) cells according to CD3/T-cell receptor (TCR) status at the genomic or protein level. The present study reports three cases of non-nasal NK-NHL with the following characteristics: an agressive clinical behavior, heterogenous morphological data evoking pleomorphic T-cell malignant lymphoma, a non-T-NK phenotype using flow cytometry, and immunochemistry. The three cases were CD56+ without membrane expression of specific T markers (CD3, CD5, and TCR). Heterogenous results were observed concerning different antigens: CD2, CD4, CD8, CD16, CD94, CD122, TiA1, perforin, and granzyme B. There was no evidence of detectable clonal TCR gene rearrangement with polymerase chain reaction. No NK activity was detected in the two tested cases, and no relation was found with EBV. Multidrug resistance investigations suggest that agressive clinical findings could be related to MDR1 gene expression as confirmed by MDR1 mRNA detection, MDR1 gene product (Pgp) expression, and a functional multidrug resistance study using rhodamine efflux by flow-cytometry.
CD56表达先前已在一些非霍奇金淋巴瘤（NHL）特征中被报道。它们主要累及鼻咽部，与爱泼斯坦-巴尔病毒（EBV）相关，并且根据基因组或蛋白质水平的CD3/T细胞受体（TCR）状态可被分类为T或非T自然杀伤（NK）细胞。本研究报告了3例非鼻型NK-NHL，具有以下特征：侵袭性临床行为、引发多形性T细胞恶性淋巴瘤的异质性形态学数据、使用流式细胞术和免疫化学的非T-NK表型。这3例病例CD56阳性，无特异性T标志物（CD3、CD5和TCR）的膜表达。关于不同抗原观察到异质性结果：CD2、CD4、CD8、CD16、CD94、CD122、TiA1、穿孔素和颗粒酶B。聚合酶链反应未发现可检测到的克隆性TCR基因重排证据。在两个检测病例中未检测到NK活性，且未发现与EBV有关。多药耐药性研究表明，侵袭性临床发现可能与MDR1基因表达有关，MDR1 mRNA检测、MDR1基因产物（Pgp）表达以及使用流式细胞术通过罗丹明外排进行的功能性多药耐药性研究证实了这一点。
