Osborn E C, Mackenzie J C, Rigby G V, Wilton A, Sugden P L
Am J Physiol. 1977 Oct;233(4):H527-34. doi: 10.1152/ajpheart.1977.233.4.H527.
The acceptability of radioimmunoassay to determine the levels of compounds antigenic to anti-angiotensin antibodies, in unprocessed methanolic blood extracts, was established for sheep blood. This approach was used to follow the clearance of antigenic compounds after administration of angiotensins I and II and fragments of angiotensin II in anesthetized sheep. The organs supplied by the systemic circulation and also the lungs effectively removed angiotensin I, but the removal of octapeptide occurred only in the peripheral tissues. The blood concentrations of compounds reacting with the anti-angiotensin II antibody always increased with passage of angiotensin I through the pulmonary circulation but not with passage of angiotensin II. The results indicate that factors other than efficiency of removal by the tissue is important in establishing blood levels. The sites of administration and of sampling were shown to be important in relation to ratios of the concentrations of antigenic material. There was a similar uptake of both hormones in the kidney; the relative inability of angiotensin I to reduce renal blood flow, therefore, does not result from a failure of uptake.
已确定放射免疫分析法用于测定未经处理的甲醇血提取物中对抗血管紧张素抗体具有抗原性的化合物水平在绵羊血液中的可接受性。该方法用于追踪麻醉绵羊注射血管紧张素I、II及血管紧张素II片段后抗原性化合物的清除情况。体循环供血的器官以及肺能有效清除血管紧张素I，但八肽仅在周围组织中被清除。与抗血管紧张素II抗体反应的化合物的血药浓度总是随着血管紧张素I通过肺循环而升高，但不随血管紧张素II通过而升高。结果表明，除了组织清除效率之外，其他因素对于确定血药水平也很重要。给药部位和取样部位被证明与抗原物质浓度比有关。两种激素在肾脏中的摄取情况相似；因此，血管紧张素I相对无法降低肾血流量并非由于摄取失败。
