Acar H, Stewart J, Connor M J
Department of Medical Genetics, University of Selçuk, Konya, Turkey.
Cancer Genet Cytogenet. 1997 Apr;94(2):75-8. doi: 10.1016/s0165-4608(96)00167-7.
In this study, patients with Philadelphia (Ph) negative and positive chronic myelogenous leukemia (CML) were analyzed by unicolor- and dual color- (DC) fluorescence in situ hybridization (FISH) using abl and bcr cosmid probes. Unicolor- and DC-FISH analysis revealed a BCR-ABL fusion in a Ph-negative patient. DC-FISH to interphase nuclei revealed the BCR-ABL fusion, even though no metaphases were available for metaphase-FISH and conventional cytogenetic analysis. In the remaining cases, the findings of FISH were in agreement with the cytogenetic results.
在本研究中，使用abl和bcr黏粒探针，通过单色和双色荧光原位杂交（FISH）对费城染色体（Ph）阴性和阳性慢性髓性白血病（CML）患者进行分析。单色和双色FISH分析在一名Ph阴性患者中检测到BCR-ABL融合。对间期核进行双色FISH检测发现了BCR-ABL融合，尽管没有中期细胞可用于中期FISH和传统细胞遗传学分析。在其余病例中，FISH结果与细胞遗传学结果一致。
