Höckerfelt U, Kjörell U, Malm V, Henriksson R, Franzén L, Forsgren S
Department of Anatomy, Umeå University, Sweden.
Regul Pept. 1997 Jan 29;68(2):83-9. doi: 10.1016/s0167-0115(96)02107-6.
In the present study, the immunohistochemical expression of vasoactive intestinal polypeptide (VIP) in the parotid gland of rats exposed to fractionated irradiation was examined. VIP concentration was analyzed by radioimmunoassay (RIA). Irradiation lead to a marked increase in the immunohistochemical expression of VIP in the innervation of the gland parenchyme. VIP-like immunoreactivity (LI) frequently coexisted with SP (substance P)-LI in these nerve fibers. The pattern of VIP-innervation in association with large ducts and blood vessel walls was unchanged. RIA analysis revealed a more than three-fold elevation in VIP content in the gland in response to irradiation. The increase in VIP immunoreaction and VIP content was seen at examination ten days after cessation of a five-day treatment with a total dose of 30-40 Gray. The upregulation of VIP may be related to changes in the production of neurotrophic factors and to an increased demand for a potentiation of secretagogue effects of SP.
在本研究中，检测了分次照射大鼠腮腺中血管活性肠肽（VIP）的免疫组化表达。通过放射免疫分析（RIA）测定VIP浓度。照射导致腺实质神经支配中VIP的免疫组化表达显著增加。在这些神经纤维中，VIP样免疫反应性（LI）常与P物质（SP）-LI共存。与大导管和血管壁相关的VIP神经支配模式未改变。RIA分析显示，照射后腺体中VIP含量升高了三倍多。在总剂量为30 - 40戈瑞的五天治疗停止十天后的检查中，观察到VIP免疫反应和VIP含量增加。VIP的上调可能与神经营养因子产生的变化以及对增强SP促分泌作用的需求增加有关。
