Farbman A I
Anat Rec. 1977 Oct;189(2):187-99. doi: 10.1002/ar.1091890206.
Presumptive olfactory mucosa was excised from the heads of rat fetuses in the eleventh and twelfth days of gestation and explanted in organ cultures. At the time of explanation, the presumptive olfactory cells were recognizable by their long narrow apical processes and basally located nuclei. However at this stage they were in an early phase of differentiation as indicated by the large numbers of free ribosomes and virtual absence of microtubules and cilia in the apical cytoplasm. After three to eight days in culture, there was a progressive increase in the total number of cells in the epithelium. Differentiation in olfactory receptor cells was detectable by the appearance in the apical processes of axially oriented microtubules and centrioles or basal bodies, some of which generated cilia. At their basal ends, the cytoplasm narrowed into axons and bundles of these axons, arranged in the unique manner of olfactory nerve axons, entered the connective tissue. Olfactory receptor cells, as defined by morphological criteria, differentiated under suitable organ culture conditions in the absence of any tissue from the central nervous system.
在妊娠第11天和第12天，从大鼠胎儿头部切除假定的嗅黏膜，并将其接种于器官培养物中。在接种时，假定的嗅细胞可通过其细长的顶端突起和位于基部的细胞核识别。然而，在这个阶段，它们处于分化的早期阶段，表现为大量游离核糖体，顶端细胞质中几乎没有微管和纤毛。培养三到八天后，上皮细胞总数逐渐增加。通过顶端突起中轴向排列的微管和中心粒或基体的出现，可以检测到嗅觉受体细胞的分化，其中一些会产生纤毛。在其基部末端，细胞质变窄形成轴突，这些轴突束以嗅觉神经轴突独特的方式排列，进入结缔组织。根据形态学标准定义的嗅觉受体细胞，在没有来自中枢神经系统任何组织的情况下，在合适的器官培养条件下分化。
