Yoshihara T, Honma S, Mitome M, Honma K
Department of Physiology, Hokkaido University School of Medicine, Sapporo, Japan.
Neurosci Lett. 1997 Jan 31;222(2):95-8. doi: 10.1016/s0304-3940(97)13353-5.
Free-running period of the feeding-associated circadian rhythm was assessed in suprachiasmatic nucleus (SCN) lesioned rats under three different conditions using a feeding-fasting (FF) paradigm; restricted feeding (RF) with meal intervals of 24 h (T = 24 h) under 24 h light-dark cycle (LD), RF with T = 24 h under continuous dim light (dim LL), and RF with T = 25 h under dim LL. After the termination of RF, the rats were subjected to FF regimen five times repeatedly, in which food and water were available for 7 days, followed by total food deprivation for 3 days with free-access to water. Free-running period, which was measured with reference to the prefeeding activity peak during food deprivation, was very close to 24 h and was not different under three conditions. It is concluded that the feeding-associated circadian rhythm has a major period close to 24 h, which is not affected either by light conditions nor by meal intervals.
使用进食-禁食（FF）范式，在三种不同条件下，对视交叉上核（SCN）损伤的大鼠进食相关昼夜节律的自由运行周期进行了评估；在24小时明暗循环（LD）下，进食间隔为24小时（T = 24小时）的限时进食（RF），在持续昏暗光照（昏暗LL）下T = 24小时的RF，以及在昏暗LL下T = 25小时的RF。在RF结束后，大鼠反复接受5次FF方案，即食物和水供应7天，然后完全剥夺食物3天，同时可自由饮水。以食物剥夺期间喂食前活动高峰为参照测量的自由运行周期非常接近24小时，且在三种条件下无差异。得出的结论是，进食相关昼夜节律的主要周期接近24小时，不受光照条件或进食间隔的影响。
