Hedin L, Eriksson A
Department of Physiology, Göteborg University, Sweden.
Prostaglandins. 1997 Feb;53(2):91-106. doi: 10.1016/s0090-6980(97)00002-6.
The inducible form of prostaglandin endoperoxide-2 (PGS-2) is transiently induced by activators of the protein kinase A and protein kinase C systems in rat preovulatory (PO) granulosa cells. This induction is suggested to play an important role in the ovulatory process, which shares many of the characteristics of an inflammatory reaction. The purpose of the present study was to explore the role of progesterone (P4) as an "anti-inflammatory" steroid for the regulation of PGS-2 and the synthesis of prostaglandins in the PO follicle. Isolated rat PO follicles were preincubated with different amounts of exogenous P4 before the addition of luteinizing hormone (LH) and 3-isobutyl-1-methylxanthine (IBMX) (LH + 1). Medium levels of prostaglandin E2 (PGE2) were measured by RIA and the protein contents of PGS-1 and PGS-2 were determined by immunoblotting. LH + I. Both the content of PGS-2 and the synthesis of PGE2 were decreased. The content of PGS-1 demonstrated only minor changes in response to P4. These results showed a dual regulation of PGS-2 in the rat PO follicle with both stimulatory and inhibitory pathways. One of the "anti-inflammatory" actions exerted by P4 in the present study was to reduce the expression of PGS-2 and the follicular production of prostaglandins. This action might be of importance for restriction and control of the inflammatory response in the ovulatory process in vivo.
前列腺素内过氧化物-2（PGS-2）的诱导型在大鼠排卵前（PO）颗粒细胞中由蛋白激酶A和蛋白激酶C系统的激活剂短暂诱导。这种诱导被认为在排卵过程中起重要作用，排卵过程具有许多炎症反应的特征。本研究的目的是探讨孕酮（P4）作为一种“抗炎”类固醇对PO卵泡中PGS-2的调节和前列腺素合成的作用。在添加促黄体生成素（LH）和3-异丁基-1-甲基黄嘌呤（IBMX）（LH + 1）之前，将分离的大鼠PO卵泡与不同量的外源性P4进行预孵育。通过放射免疫分析法（RIA）测定前列腺素E2（PGE2）的培养基水平，并通过免疫印迹法测定PGS-1和PGS-2的蛋白质含量。LH + 1。PGS-2的含量和PGE2的合成均降低。PGS-1的含量对P4的反应仅显示出微小变化。这些结果表明大鼠PO卵泡中PGS-2存在刺激和抑制途径的双重调节。本研究中P4发挥的“抗炎”作用之一是降低PGS-2的表达和卵泡中前列腺素的产生。这种作用可能对体内排卵过程中炎症反应的限制和控制具有重要意义。
