槲皮素与热处理对低HSP70表达的淋巴细胞系（OZ）的协同细胞毒性作用。

Synergistic cytotoxic effect of quercetin and heat treatment in a lymphoid cell line (OZ) with low HSP70 expression.

作者信息

Fujita M, Nagai M, Murata M, Kawakami K, Irino S, Takahara J

机构信息

First Department of Internal Medicine, Kagawa Medical University, Kita-gun, Japan.

出版信息

Leuk Res. 1997 Feb;21(2):139-45. doi: 10.1016/s0145-2126(96)00080-x.

DOI:10.1016/s0145-2126(96)00080-x

PMID:9112431

Abstract

Heat shock protein 70 (HSP70) protects cells from various injurious stimuli and is important in cell growth and differentiation. However, its expression in leukemia cells has not been analyzed systematically. We, therefore, investigated the expression of HSP70 in various types of leukemia cells and hematopoietic cell lines. Immunoblot analysis revealed that HSP72/73 were expressed at low levels in the acute lymphocytic leukemia cells and lymphoid cell lines examined. However, heat (43 C for 2 h) preferentially augmented HSP72/73 expression in lymphoid cells. This induction was partially blocked by the treatment with quercetin (10 microM for 24 h). Finally, heat shock following quercetin treatment synergistically induced apoptosis in a lymphoid cell line (OZ). These observations suggest the possibility of selective purging of lymphocytic leukemia cells by combination with quercetin and heat.

摘要

热休克蛋白70（HSP70）可保护细胞免受各种损伤刺激，在细胞生长和分化中起重要作用。然而，其在白血病细胞中的表达尚未得到系统分析。因此，我们研究了HSP70在各种类型白血病细胞和造血细胞系中的表达。免疫印迹分析显示，在所检测的急性淋巴细胞白血病细胞和淋巴样细胞系中，HSP72/73表达水平较低。然而，热刺激（43℃，2小时）优先增强淋巴样细胞中HSP72/73的表达。槲皮素（10微摩尔，24小时）处理可部分阻断这种诱导作用。最后，槲皮素处理后的热休克协同诱导淋巴样细胞系（OZ）凋亡。这些观察结果提示，联合使用槲皮素和热刺激可能选择性清除淋巴细胞白血病细胞。