García-Arata M I, Gerner-Smidt P, López-Brea M
Department of Clinical Microbiology, Hospital Ramón y Cajal, Madrid, Spain.
APMIS. 1997 Feb;105(2):131-8.
An epidemiological survey of the increase in Acinetobacter species isolates occurring in the intensive care unit of a Spanish teaching hospital during 1993 and 1994 was carried out. Different laboratory methods were used to find out, whether there was a genetic linkage. The isolates were divided into three main groups according to the resistance patterns to 11 drugs. Using API 20NE biotyping, eight different types were found. The two most common contained 20 and 11 isolates, respectively. Five different plasmid profile types were observed, although plasmids were only demonstrated in 40% of the isolates. Ribotyping with EcoRI, SalI and ClaI enzymes revealed 10, 9, and 8 different patterns, respectively. In total, 15 different ribotypes were identified using these three enzymes. Twenty-one isolates belonged to exactly the same ribotype, and 13 were associated with two highly related ribotypes. In the first ribotype, only five isolates harboured plasmids. The ribotyping method produced 100% typability and ribotypes were easy to compare; it also had taxonomic value. Ribotyping allowed us to determine the genetic linkage between Acinetobacter isolates recovered from ICU patients.
对一家西班牙教学医院重症监护病房1993年至1994年期间不动杆菌属分离株增加情况进行了一项流行病学调查。采用了不同的实验室方法来查明是否存在基因联系。根据对11种药物的耐药模式，将分离株分为三个主要组。使用API 20NE生物分型法，发现了8种不同类型。最常见的两种类型分别包含20株和11株分离株。观察到5种不同的质粒图谱类型，尽管仅在40%的分离株中证实存在质粒。用EcoRI、SalI和ClaI酶进行核糖体分型分别揭示了10种、9种和8种不同模式。使用这三种酶总共鉴定出15种不同的核糖体类型。21株分离株属于完全相同的核糖体类型，13株与两种高度相关的核糖体类型相关。在第一种核糖体类型中，只有5株分离株含有质粒。核糖体分型方法产生了100%的分型能力，且核糖体类型易于比较；它还具有分类学价值。核糖体分型使我们能够确定从ICU患者中分离出的不动杆菌之间的基因联系。
