Maeland J A, Piene M, Müller L I
Department of Microbiology, School of Medicine, Norwegian University of Science and Technology, Trondheim.
APMIS. 1997 Feb;105(2):157-60.
Culture isolation and identification of Helicobacter pylori represents a considerable work load in clinical microbiology. The aim of this study was to test if antibody-mediated bacterial agglutination could be used for rapid identification of H. pylori. Rabbit antiserum against H. pylori strain I and against another strain, H. pylori 330, which was very weakly agglutinated (1+) by anti-H. pylori I serum, were mixed and used in a slide agglutination test. Of 107 consecutive clinical isolates tested, 101 (94%) strains showed 2+ or 3+ reaction using the antiserum mixture, whereas 6 (6%) strains could not be evaluated owing to autoagglutinability. Bacteria of a variety of other species, including Campylobacter spp., showed no agglutination with the antiserum mixture. The results support the notion that reliable identification of the majority of cultured H. pylori strains should be possible in less than 3 min by agglutination testing.
幽门螺杆菌的培养分离和鉴定在临床微生物学中是一项相当繁重的工作。本研究的目的是测试抗体介导的细菌凝集是否可用于快速鉴定幽门螺杆菌。将抗幽门螺杆菌菌株I的兔抗血清与另一种菌株幽门螺杆菌330（抗幽门螺杆菌I血清对其凝集反应非常弱，为1+）的兔抗血清混合，并用于玻片凝集试验。在连续检测的107株临床分离株中，101株（94%）菌株使用抗血清混合物显示2+或3+反应，而6株（6%）菌株由于自身凝集性无法评估。包括弯曲杆菌属在内的多种其他菌种的细菌与抗血清混合物无凝集反应。结果支持这样一种观点，即通过凝集试验在不到3分钟的时间内对大多数培养的幽门螺杆菌菌株进行可靠鉴定应该是可行的。
