细胞色素P450 4A和2E1在人肾微粒体中的表达。

Cytochrome P450 4A and 2E1 expression in human kidney microsomes.

作者信息

Amet Y, Berthou F, Fournier G, Dréano Y, Bardou L, Clèdes J, Ménez J F

机构信息

Laboratoire de Biochimie-Nutrition, Faculté de Médecine, Brest, France.

出版信息

Biochem Pharmacol. 1997 Mar 21;53(6):765-71. doi: 10.1016/s0006-2952(96)00821-0.

DOI:10.1016/s0006-2952(96)00821-0

PMID:9113097

Abstract

Laurate and arachidonate omega and (omega-1)-hydroxylase activities, cytochrome P450 2E1 (CYP2E1), and CYP4A content were measured in 18 human kidney microsomal samples. The rates of laurate and arachidonate were found to be very different from those measured in human liver samples, with a laurate omega/omega-1 ratio of approximately 22 in human kidney vs 0.75 in human liver. Immunoblot analysis of the 18 human kidney microsomal samples identified 1 CYP4A electrophoretic band, but CYP2E1 was not detectable in human kidney, contrary to liver. Laurate and arachidonate omega-hydroxylase activities were significantly correlated with CYP4A content (r = 0.86 and 0.75, respectively). Polyclonal antirat CYP2E1 antibody did not affect omega-hydroxylase activity, whereas the polyclonal antirat CYP4A1 antibody inhibited it by 60%. These results suggest that, in contrast to other species, human kidney microsomes do not contain significant amounts of CYP2E1, but possess CYP4A and fatty acid omega-hydroxylase activity.

摘要

在18份人肾微粒体样本中检测了月桂酸和花生四烯酸的ω和（ω-1）羟化酶活性、细胞色素P450 2E1（CYP2E1）以及CYP4A含量。发现月桂酸和花生四烯酸的速率与人肝样本中的测量值有很大差异，人肾中月桂酸的ω/ω-1比值约为22，而人肝中为0.75。对18份人肾微粒体样本进行免疫印迹分析，鉴定出1条CYP4A电泳条带，但与人肝相反，在人肾中未检测到CYP2E1。月桂酸和花生四烯酸的ω-羟化酶活性与CYP4A含量显著相关（分别为r = 0.86和0.75）。多克隆抗大鼠CYP2E1抗体不影响ω-羟化酶活性，而多克隆抗大鼠CYP4A1抗体可将其抑制60%。这些结果表明，与其他物种不同，人肾微粒体不含大量CYP2E1，但具有CYP4A和脂肪酸ω-羟化酶活性。