Lankiewicz S, Gisselmann G, Hatt H
Lehrstuhl für Zellphysiologie, Ruhr-Universität Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany.
Nucleic Acids Res. 1997 May 15;25(10):2037-8. doi: 10.1093/nar/25.10.2037.
RACE (rapid amplification of cDNA ends) is commonly used for identification and isolation of 3'and 5'termini of cDNA. We developed an improvement of the RACE-method that allows the enrichment of wanted fragments. The important new feature is the purification of the amplified products by biotinylated oligonucleotides that hybridize internally. Hybrids are isolated by streptavidin coated magnetic particles.
cDNA末端快速扩增（RACE）通常用于鉴定和分离cDNA的3'和5'末端。我们对RACE方法进行了改进，使其能够富集所需片段。重要的新特性是通过内部杂交的生物素化寡核苷酸对扩增产物进行纯化。通过链霉亲和素包被的磁性颗粒分离杂交体。
